中国中药杂志2026，Vol.51Issue(9)：2676-2688,13.DOI:10.19540/j.cnki.cjcmm.20260202.204
基于色谱-质谱联用技术的咽立爽口含滴丸在大鼠胆汁、粪便、尿液中的代谢、排泄研究
Study on metabolism and excretion of Yanlishuang Oral Dripping Pills in rat bile,feces,and urine based on chromatography-mass spectrometry technology
摘要
Abstract
To delineate the in vivo metabolism and excretion of Yanlishuang Oral Dripping Pills in rats,an integrated analytical strategy was implemented through chromatography-mass spectrometry technology.Biological samples(bile,feces,and urine)were collected before and after oral administration of Yanlishuang Oral Dripping Pills.Processed samples were analyzed by gas chromatography-mass spectrometry(GC-MS)and ultra-high performance liquid chromatography-tandem mass spectrometry(UHPLC-MS/MS).Through systematic comparison of retention time,mass-to-charge ratios,and characteristic fragment ions,a total of 4 prototype compounds and 30 metabolites were identified across biological matrices,including 18 metabolites in bile,13 in feces,and 24 in urine.Primary metabolic pathways involved hydrolysis,hydrogenation,methylation,sulfation,and glucuronidation.Quantitative analysis further determined cumulative excretion rates of prototype components(borneol,menthol,camphor,and glycyrrhizic acid)and their metabolites(borneol-2-O-glucuronide,menthol-1-O-glucuronide,glycyrrhetinic acid,and glycyrrhetinic acid-3-O-β-D-glucuronide)in bile,feces,and urine.This study systematically clarified the in vivo metabolism and excretion of Yanlishuang Oral Dripping Pills,providing critical experimental evidence for further clinical applications.关键词
咽立爽口含滴丸/代谢途径/排泄研究/色谱-质谱联用技术Key words
Yanlishuang Oral Dripping Pills/metabolic pathways/excretion study/chromatography-mass spectrometry technology引用本文复制引用
彭玥,袁红飞,张丽,徐贵萍,李鸿迪,郑林,迟明艳..基于色谱-质谱联用技术的咽立爽口含滴丸在大鼠胆汁、粪便、尿液中的代谢、排泄研究[J].中国中药杂志,2026,51(9):2676-2688,13.基金项目
贵州省科技重大专项项目(黔科合重大专项字[2024]015) （黔科合重大专项字[2024]015）
贵州省科技计划项目(黔科合支撑[2023]一般234,黔科合平台人才-GCC[2023]036/CXTD[2023]019,黔科合平台KXJZ[2025]014/CXPTXM[2025]010) （黔科合支撑[2023]一般234,黔科合平台人才-GCC[2023]036/CXTD[2023]019,黔科合平台KXJZ[2025]014/CXPTXM[2025]010）
