中国中药杂志2026，Vol.51Issue(10)：2769-2780,12.DOI:10.19540/j.cnki.cjcmm.20260209.306
生、炙黄芪不同组分对脾虚大鼠肠道菌群、短链脂肪酸代谢和免疫活性的调节作用研究
Modulatory effects of raw and honey-processed Astragali Radix fractions on intestinal microbiota,short-chain fatty acid metabolism and immune activity in rats with spleen deficiency
摘要
Abstract
This study aimed to investigate the effects of total saponins,total flavonoids,and total polysaccharides from raw and honey-processed Astragali Radix on intestinal microbiota,short-chain fatty acid metabolism,and immune activity in rats with spleen deficiency.A spleen deficiency model was established using a combination of irregular diet,bitter-cold purgative administration and excessive fatigue.High-throughput 16S rRNA sequencing was employed to analyze the modulatory effects of different fractions from raw and honey-processed Astragali Radix on the structural diversity of intestinal microbiota in spleen-deficient rats.Headspace gas chromatography-mass spectrometry was used to determine the modulatory effects of these fractions on the metabolism of short-chain fatty acids.ELISA was employed to assess the effects of raw and honey-processed Astragali Radix on specific and non-specific immune activities of spleen-deficient rats.The results demonstrated that the total flavonoids,total saponins,and total polysaccharides from raw and honey-processed Astragali Radix significantly restored the abundance of the harmful bacterial genus Helicobacter in the intestines of spleen-deficient rats.Moreover,except for the total polysaccharides from both raw and honey-processed Astragali Radix,all other treatment groups also significantly reduced the abundance of the harmful bacterial genus Sutterella(P＜0.05).The fractions from raw and honey-processed Astragali Radix were able to restore the levels of various short-chain fatty acids in the colon of spleen-deficient rats.Notably,the total polysaccharides from honey-processed Astragali Radix significantly outperformed those from raw Astragali Radix in increasing acetic acid content(P＜0.05).The total polysaccharides of both raw and honey-processed Astragali Radix significantly restored the levels of IL-4,IL-6,IL-10,IL-18,IgA,and IgG in the serum and colon,as well as the level of IL-2 in the serum of spleen-deficient rats(P＜0.05 or P＜0.01).The restorative effects of the processed product were superior to those of the raw product.In summary,the specific and non-specific immunomodulatory effects of the components from raw Astragali Radix increased after honey-processing,thereby potentiating its efficacy in ameliorating spleen deficiency.These findings may provide a scientific basis for elucidating the mechanism by which honey-processing enhances the qi-tonifying and middle-strengthening effects of Astragali Radix.关键词
黄芪/蜜炙/肠道菌群/短链脂肪酸/免疫活性Key words
Astragali Radix/honey-processed/intestinal microbiota/short-chain fatty acid/immune activity引用本文复制引用
李相蓉,缪翼翔,任天航,常源,刘蓬蓬..生、炙黄芪不同组分对脾虚大鼠肠道菌群、短链脂肪酸代谢和免疫活性的调节作用研究[J].中国中药杂志,2026,51(10):2769-2780,12.基金项目
中国博士后科学基金项目(2024MD763986) （2024MD763986）
国家自然科学基金项目(82003945) （82003945）
