神经药理学报2026，Vol.16Issue(2)：12-19,8.DOI:10.3969/j.issn.2095-1396.2026.02.002
基于网络药理学和转录组学分析补血通络丸抗股骨头缺血性坏死的作用机制研究
Study Regarding the Mechanism of Buxue Tongluo Pill Against Osteonecrosis of Femoral Head Based on Network Pharmacology and Transcriptomics
王丹 1李国庆 2巩密密 1刘五江 1李慧璇 1邵秀丽 1张华林1
作者信息
- 1. 陆军第八十一集团军医院,张家口,075000,中国
- 2. 张家口市第一医院,张家口,075000,中国
- 折叠
摘要
Abstract
Objective:To investigate the preventive mechanism of Buxue Tongluo pill(BTP)against osteonecrosis of femoral head(ONFH)based on network pharmacology and transcriptomics.Methods:We established an ONFH model in SD rats using high dose dexamethasone.Thirty-six rats were randomly divided into six groups,namely normal group,model group,positive group and low-dose group,middle-dose group and high-dose group.Transcriptome sequencing regarding the femoral head was conducted 4 weeks after continuous treatment.Subsequently,mechanism of action of BTP on ONFH determined by integrated network pharmacology.Results:After BTP treatment,308 genes were significant changed in expression levels,120 genes being up-regulated and 188 being down-regulated.A total of 108 key targets were targeted for selection.KEGG analysis suggested that various pathways were largely involved in the mechanisms of BTP against ONFH,such as PI3k-Akt signal pathway,cancer,cell cycle and so on.Conclusion:BTP is effective in treating ONFH through multi-components,multi-targets and multi-channels,which may be related to apoptosis mediated by PI3k-Akt signaling pathway.关键词
补血通络丸/转录组学测序/网络药理学/股骨头缺血性坏死Key words
Buxue Tongluo pill/transcriptome sequencing/network pharmacology/osteonecrosis of femoral head分类
医药卫生引用本文复制引用
王丹,李国庆,巩密密,刘五江,李慧璇,邵秀丽,张华林..基于网络药理学和转录组学分析补血通络丸抗股骨头缺血性坏死的作用机制研究[J].神经药理学报,2026,16(2):12-19,8.
