党文 1许晴 1王辰晖 1李德伟 2白晓林 2李宁1
作者信息
- 1. 沈阳药科大学 中药学院 辽宁省重大慢病中药创新药重点实验室 沈阳市中药药效物质研究与创新药开发重点实验室,辽宁 沈阳 110016
- 2. 天士力东北现代中药资源有限公司,辽宁 本溪 117200
- 折叠
摘要
Abstract
This study aimed to investigate the chemical constituents of Eupatorii Lindleyani Herba and to identify the compounds with protective effects on the lung.The petroleum ether extract of Eupatorii Lindleyani Herba was separated and purified through silica gel column chromatography,ODS column chromatography,and HPLC.The structures of the isolated compounds were identified based on their physicochemical properties and spectroscopic data.Nine compounds were obtained and identified as eupaphenolide A(1),eupaphenolide B(2),aurantiamide acetate(3),aurantiamide(4),8β-tigloiloxi-grazielia(5),(3S,6R,7R,8R)-3-hydroxy-8-acetoxysarracenyloxy germacra-1(10),4,11(13)-trien-6,12-ilide(6),eucannabinolide(7),eupalinolide G(8),and eupachinsin A(9).Compounds 1 and 2 were newly identified.The lung-protective activities of these compounds were evaluated in vitro via the MTT assay.Germacranolide-type sesquiterpenes(5-9)showed notable protective effects,among which compound 8 at 1 μmol·L-1 significantly increased cell viability,exhibiting stronger activity than the positive control licochalcone A.Compounds 6,7,and 9 at low concentrations partially reduced NNK-induced cell injury and displayed a biphasic effect characterized by protection at low concentrations and inhibition at high concentrations.These results suggest that the germacranolide-type sesquiterpenes in Eupatorii Lindleyani Herba may serve as the main active constituents responsible for its lung-protective effects,providing experimental evidence for further pharmacological research on this plant.关键词
野马追/化学成分/肺保护作用/儿茶酚脂/吉马烷型倍半萜Key words
Eupatorii Lindleyani Herba/chemical constituents/lung-protective effect/catechol ester/germacranolide-type sesqui-terpene
