中国中药杂志2026，Vol.51Issue(10)：2805-2813,9.DOI:10.19540/j.cnki.cjcmm.20260104.202
山香圆叶化学成分研究
Chemical constituents from Turpiniae Folium
摘要
Abstract
Nineteen compounds were isolated from the Chinese medicinal material Turpiniae Folium by column chromatography on normal-phase silica gel,Sephadex LH-20,and MCI,and preparative high-performance liquid chromatography.Spectroscopic methods including nuclear magnetic resonance and high-resolution mass spectrometry were employed for the structure elucidation,by which the isolated compounds were identified as(4R,5S)-dihydroxy-6,7-megastigmadien-9-one(1),dehydrovomifoliol(2),loliolide(3),lupenone(4),β-amyrone(5),oleanolic acid(6),α-amyrone(7),friedelin(8),(20S)-3-oxo-20-hydroxytaraxastane(9),β-sitosterol(10),(13S,9Z,11E)-13-hydroxy-9,11-octadecadienoic acid methyl ester(11),(9E,11E)-13-oxo-9,11-octadecadienoic acid(12),vitamin E quinone(13),3-furoic acid(14),4-hydroxyisobenzofuran-1(3H)-one(15),eugenin(16),4-hydroxybenzaldehyde(17),4-hydroxyacetophenone(18),and vanillic acid(19).Among them,compound 1 was identified as a new megastigmane-type nor-sesquiterpenoid characterized by an allene moiety,and its absolute configuration was determined by quantum chemical calculation of the ECD spectrum.Additionally,compounds 2-5,7-9,and 11-18 were isolated from the genus Turpinia for the first time.The antibacterial and anti-inflammatory activities of the compounds were evaluated by the double dilution method and the lipopolysaccharide(LPS)-induced inflammation model in mouse macrophage RAW264.7 cells,respectively.The results revealed the inhibitory effects of compounds 2 and 11-13 on nitric oxide production in LPS-induced RAW264.7 cells.关键词
山香圆叶/化学成分/抗炎活性/量子化学计算Key words
Turpiniae Folium/chemical constituent/anti-inflammatory activity/quantum chemical calculation引用本文复制引用
武舒敏,杨胜祥,杨健,贾海燕,唐菲菲,何云清,梁晓瑜,马千荔,海萍,高原,詹志来..山香圆叶化学成分研究[J].中国中药杂志,2026,51(10):2805-2813,9.基金项目
中国中医科学院科技创新工程项目(JBKY2025A06) （JBKY2025A06）
国家中医药管理局高水平中医药重点学科建设项目(ZYYZDXK-2023244) （ZYYZDXK-2023244）
中央本级重大增减支项目(2060302) （2060302）
财政部和农业农村部国家现代农业产业技术体系项目(CARS-21) （CARS-21）
香料植物资源开发与利用四川省高校重点实验室开放基金项目(22XLZ02) （22XLZ02）
宜宾学院高层次人才启航计划项目(2023QHO7) （2023QHO7）
