中国中药杂志2026，Vol.51Issue(10)：2836-2844,9.DOI:10.19540/j.cnki.cjcmm.20260206.706
双参扶正散效应物质分析及其对人肺腺癌A549细胞的机制研究
Analysis of effective substances in Shuangshen Fuzheng San and its mechanism study on human lung adenocarcinoma A549 cells
摘要
Abstract
Non-targeted TCM metabolomic techniques were employed to explore the potential effective substances of Shuangshen Fuzheng San.The inhibitory effects of Shuangshen Fuzheng San and its effective substances on human lung adenocarcinoma A549 cells in vitro were evaluated by using CCK-8 assays,colony formation assays,flow cytometric analysis of apoptosis,and Western blot analysis of apoptosis-related proteins.This study aims to explore the optimal effective substance combination,further analyze,and verify the potential action mechanisms underlying the inhibitory effects of Shuangshen Fuzheng San and its effective substances on the lung adenocarcinoma through network pharmacology analysis.The results demonstrate that ginsenoside Rg3,ginsenoside Ro,and notoginsenoside Fe can simultaneously enter into the mice's serum and lung tissues and are identified as the key effective substances of Shuangshen Fuzheng San responsible for inhibiting A549 cell proliferation.The combination of ginsenoside Rg3 and notoginsenoside Fe exhibits the strongest inhibitory effect on A549 cell proliferation and is determined to be the optimal effective substance combination.The optimal effective substance combination can inhibit clonogenic formation of A549 cells,significantly promote the apoptosis of A549 cells,and markedly upregulate the expression of apoptosis-related proteins,with the effective substance combination showing a superior effect compared with the drug-containing serum.The interleukin(IL)-17 signaling pathway is strongly associated with the inhibitory effects of Shuangshen Fuzheng San on lung adenocarcinoma.Consistently,both the optimal effective substance combination of Shuangshen Fuzheng San and drug-containing serum can significantly downregulate the expression levels of key IL-17 pathway-related proteins,including IL-17A,IL-6,and matrix metalloproteinase-9(MMP-9),in A549 cells.Collectively,ginsenoside Rg3,ginsenoside Ro,and notoginsenoside Fe are the key effective substances of Shuangshen Fuzheng San.The combination of ginsenoside Rg3 and notoginsenoside Fe constitutes the optimal effective substance combination of Shuangshen Fuzheng San,which together with drug-containing serum,inhibits lung adenocarcinoma progression via the IL-17 signaling pathway and exhibits superior inhibitory effects on lung adenocarcinoma in vitro compared with drug-containing serum.关键词
肺腺癌/双参扶正散/中医药/效应物质/人参皂苷Rg3/三七皂苷FeKey words
lung adenocarcinoma/Shuangshen Fuzheng San/TCM/effective substance/ginsenoside Rg3/notoginsenoside Fe引用本文复制引用
李玥,李卫东,花宝金,刘瑞,罗钺,胡佳奇,高业博,耿良,雷泽杭,于惠博,王林枫,张曦文..双参扶正散效应物质分析及其对人肺腺癌A549细胞的机制研究[J].中国中药杂志,2026,51(10):2836-2844,9.基金项目
国家自然科学基金项目(82274609,82405518) （82274609,82405518）
四大慢病重大专项(2023ZD0502500,2023ZD0502502) （2023ZD0502500,2023ZD0502502）
中国博士后科学基金面上项目(2025M773954) （2025M773954）
中国中医科学院科技创新工程创新团队项目(CI2021B009) （CI2021B009）
国家中医药传承创新团队项目(ZYYCXTD-C-202205) （ZYYCXTD-C-202205）
国家中医药管理局岐黄学者支持项目 （）
