中国药科大学学报2026，Vol.57Issue(3)：332-340,9.DOI:10.11665/j.issn.1000-5048.2025080603
雾化对不同性质活性成分皮肤递送行为的影响及机制
Effects and mechanisms of atomization on the transdermal delivery behavior of active ingredients with diverse properties
摘要
Abstract
This study systematically examined the effects of atomization as a transdermal permeation enhancer on the transdermal delivery of active ingredients with different physicochemical properties and investigated the underlying mechanisms.Porcine ear skin served as an in vitro model,and skin permeation experiments were conducted using Franz diffusion cells.Receptor fluids were collected at specific time points,and samples of the stratum corneum and viable epidermis-dermis were separated and prepared.Quantitative and visualization methods,including high-performance liquid chromatography,fluorescence derivatization,and confocal laser scanning microscopy,were used to compare skin retention and transdermal permeation of adenosine(AD),tranexamic acid(TXA),calcein(CAL),and tocopheryl acetate(TA)under atomized and non-atomized conditions.Additionally,transepidermal water loss(TEWL)measurements and attenuated total reflectance-Fourier transform infrared spectroscopy(ATR-FTIR)were employed to analyze the mechanism behind the penetration enhancement caused by atomization.The findings showed that atomization increased skin r etention within the stratum corneum and viable epidermis-dermis,as well as the transdermal permeation of all four active ingredients to varying degrees.The most significant enhancement was observed for TXA,which has a relatively low molecular weight and moderate lipophilicity,while the enhancement effects for highly hydrophilic AD and highly lipophilic TA were comparatively limited;however,atomization significantly increased the skin retention of TA.TEWL results indicated that skin permeability increased within 4 hours after atomization and largely recovered within 8 hours.ATR-FTIR analysis suggested that atomization may facilitate transdermal delivery by disrupting the lipid order and keratin conformation in the stratum corneum.This study provides theoretical support and experimental references for the advancement of atomization transdermal delivery technology,as well as the design and development of related atomization products.关键词
雾化给药/经皮促渗/腺苷/传明酸/钙黄绿素/生育酚乙酸酯/经皮水分散失/傅里叶变换红外光谱Key words
atomization transdermal delivery/transdermal penetration enhancement/adenosine/tranexamic acid/calcein/tocopherol acetate/transepidermal water loss/Fourier transform infrared spectroscopy分类
医药卫生引用本文复制引用
陈雅婷,韩瑞芳,尚靖,李湘,崔斌,宋文婷..雾化对不同性质活性成分皮肤递送行为的影响及机制[J].中国药科大学学报,2026,57(3):332-340,9.基金项目
国家自然科学基金项目(No.82374049) This study was supported by the National Natural Science Foundation of China(No.82374049) （No.82374049）
