中国药科大学学报2026，Vol.57Issue(3)：360-368,9.DOI:10.11665/j.issn.1000-5048.2025120801
间充质基质细胞逆转子宫内膜纤维化的机制研究
Mechanism of mesenchymal stromal cells reversing fibrosis of the uterine endometrium after injury
摘要
Abstract
To investigate the effect of mesenchymal stromal cells(MSCs)on the fibrosis progression and endometrial regeneration in mouse models of endometrial injury,a mouse endometrial injury model was established by physical scraping combined with lipopolysaccharide(LPS)chemical induction,and the endometrium morphology and collagen deposition were evaluated by HE and Masson staining after unilateral administration of MSCs.RT-qPCR and immunofluorescence were used to detect the expression of epithelial markers Epcam and collagen I(Col1).Primary endometrial epithelial cells were isolated to assess p21 expression.Fibrosis-related genes and signaling pathways were validated using transcriptome sequencing and RT-qPCR.The experimental results showed that MSCs increased endometrial thickness and number of glands(P<0.05),up-regulated the expression of Epcam(P<0.01),while significantly reducing uterine collagen fiber deposition(P<0.05)and the expression of p21 in primary epithelial cells(P<0.000 1).Transcriptomic analysis revealed that MSCs attenuated collagen deposition by modulating genes involved in the TGF-β signaling pathway.This study elucidates that MSCs facilitate uterine repair by reversing endometrial fibrosis and promoting epithelial regeneration,and their role is related to the regulation of the TGF-β/SMAD pathway and suppression of aberrant c ollagen accumulation.关键词
子宫内膜损伤/间充质基质细胞/胶原纤维/TGF-β/SMAD通路Key words
endometrial injury/mesenchymal stromal cells/collagen fiber/TGF-β/SMAD pathway分类
医药卫生引用本文复制引用
李诗琪,党玥,周青青,尚鑫,刘嘉莉..间充质基质细胞逆转子宫内膜纤维化的机制研究[J].中国药科大学学报,2026,57(3):360-368,9.基金项目
国家自然科学基金项目(No.82173882) （No.82173882）
中国科协青年人才托举项目(2023QNRC001) （2023QNRC001）
江苏省自然科学基金项目(BK20220149) This study was supported by the National Natural Science Foundation of China(No.82173882) （BK20220149）
the Young Elite Scientists Sponsorship Program of China Association for Science and Technology(2023QNRC001) （2023QNRC001）
and the Natural Science Foundation of Jiangsu Province(BK20220149) （BK20220149）
