作物杂志Issue(3)：155-162,8.DOI:10.16035/j.issn.1001-7283.2026.03.021
干旱促进ABA积累以诱导青稞HVA1基因表达
Drought Promotes ABA Accumulation to Induce HVA1 Gene Expression in Hulless Barley
摘要
Abstract
Using hulless barley cultivar Kunlun 12 as experimental material,PEG 6000 was used to simulate drought stress,and treatments with abscisic acid(ABA)and the ABA biosynthesis inhibitor fluridone were employed to analyze the relationship between HVA1 gene expression and drought and ABA.The results showed that as the concentration of PEG 6000 pretreatment increased,leaf relative moisture content decreased,relative electrical conductivity decreased first and then increased,and ABA content and HVA1 gene relative expression increased first and then decreased.As the duration of 15%PEG 6000 pretreatment increased,leaf relative moisture content decreased,relative electrical conductivity increased,and ABA content and HVA1 gene relative expression first increased and then decreased.Compared with 15%PEG 6000 pretreatment alone,the combination of 15%PEG 6000 and fluridone significantly decreased endogenous ABA content and HVA1 gene relative expression,but the decrease of HVA1 gene relative expression was greater than that of ABA content.The HVA1 gene relative expression under the combination of ABA pretreatment and 15%PEG 6000 treatment was significantly lower than that of either treatment alone,showing no additive effect.In conclusion,leaf relative moisture content and electrical conductivity can be used to effectively evaluate the drought response of hulless barley.Drought stress promotes ABA accumulation,thereby inducing HVA1 gene expression.HVA1 gene expression under drought stress is regulated by other pathways in addition to endogenous ABA.关键词
青稞/干旱胁迫/脱落酸/氟啶酮/HVA1基因Key words
Hulless barley/Drought stress/Abscisic acid/Fluridone/HVA1 gene引用本文复制引用
车广惠,胡倩,姚有华,吴昆仑,丁宝军,姚晓华..干旱促进ABA积累以诱导青稞HVA1基因表达[J].作物杂志,2026,(3):155-162,8.基金项目
青海省青稞育种联合攻关项目(2025) （2025）
国家大麦(青稞)产业技术体系专项(CARS-05) （青稞）
