作物杂志Issue(3)：216-224,9.DOI:10.16035/j.issn.1001-7283.2026.03.028
淹水深度对水稻胚芽鞘伸长的影响及其生理机制
Effects of Flooding Depth on Coleoptile Growth in Rice and the Underlying Physiological Mechanisms
摘要
Abstract
The screening,utilization,and investigation of the physiological mechanisms underlying anaerobic germination tolerance germplasm resources are fundamental to developing new rice varieties suitable for direct seeding.To simply and efficiently assess the anaerobic germination tolerance of germplasm resources,this study employed coleoptile length as the primary screening criterion to assess hypoxia tolerance across 432 rice accessions under 0.5(control),5.0 and 10.0 cm flooding conditions.The results showed that,under flooding stress,coleoptile length and seed survival rate exhibited substantial genetic variation,with japonica cultivars demonstrating significantly longer coleoptiles compared to indica cultivars.Ten germplasm resources with strong anaerobic germination tolerance were identified through coleoptile length and hypoxia tolerance index.Among these,the japonica rice varieties Putaohuang and Huangpinuo,as well as the indica rice varieties Esiniu and BoB,exhibited superior hypoxia resistance during germination.Additionally,the coleoptile lengths of japonica rice varieties Putaohuang and Huangpinuo were found to be longer than those of indica rice varieties Esiniu and BoB under flooding treatments.Physiological analyses revealed that the contents of abscisic acid(ABA),gibberellic acid(GA3),indole-3-acetic acid(IAA),and the activities of α-amylase(α-AMY)and alcohol dehydrogenase(ADH)were significantly higher than the control(0.5 cm)under flooding stress,and α-AMY activity,GA3 and IAA contents of japonica rice varieties were higher than that of indica rice varieties.Correlation analysis showed that under flooding treatment,the α-AMY activity,GA3 and IAA contents of four varieties were positively correlated with coleoptile length(r>0.9).The above results showed that ABA,GA3,IAA and ROS contents and α-AMY and ADH activity promote the elongation of coleoptiles,while α-AMY activity,GA3 and IAA contents play more important positive roles in the elongation of coleoptile under flooding stress,and higher α-AMY activity,GA3 and IAA contents gave japonica varieties more low-oxygen germination tolerance.关键词
水稻/淹水深度/耐低氧萌发/胚芽鞘/种质资源/生理机制Key words
Rice/Flooding depth/Anaerobic germination tolerance/Coleoptile/Germplasm resources/Physiological mechanisms引用本文复制引用
刘冬,王亚,李君霞,马梦娟,张骐飞,李明,付景,王越涛,王付华,尹海庆..淹水深度对水稻胚芽鞘伸长的影响及其生理机制[J].作物杂志,2026,(3):216-224,9.基金项目
河南省重点研发专项(231111110500) （231111110500）
河南省农业科学院自主创新项目(2025ZC22) （2025ZC22）
河南省农业科学院科技创新团队项目(2025TD16) （2025TD16）
河南省农业科学院基础性科研项目(2025JC04) （2025JC04）
