中国中药杂志2026，Vol.51Issue(9)：2506-2514,9.DOI:10.19540/j.cnki.cjcmm.20260305.401
代谢组联合转录组分析探讨不同品种菊花萜类成分差异形成机制
Mechanism of differences in terpenoids bewteen different varieties of Chrysanthemi Flos by integrated metabolomics and transcriptomcis analyses
摘要
Abstract
This study compared the terpenoids among different varieties of Chrysanthemi Flos and explored the molecular mechanisms underlying their accumulation,aiming to provide a basis for elucidating the material basis responsible for the differences in taste,flavor,and efficacy among different varieties.Secondary metabolites of two Chrysanthemi Flos varieties—"Hangju"and"Huaiju"—were analyzed by metabolomics.Key genes were identified from the transcriptome data,and gene expression levels were determined by qRT-PCR.A total of 21 terpenoid-related differential metabolites were identified in"Hangju"and"Huaiju"via metabolomics analysis,including eucalyptol,camphor,γ-terpinene,and α-terpineol.Transcriptomics analysis yielded 21 differentially expressed genes associated with terpenoid biosynthesis.Correlation analysis revealed that ten genes had significantly positive correlations with multiple highly accumulated differential terpenoids in"Huaiju".The qRT-PCR validation showed that the expression trends of the eight enzyme genes involved in terpenoid biosynthesis were consistent with those obtained from transcriptome sequencing in"Hangju"and"Huaiju".This study elucidates the differences in terpenoids and their accumulation mechanisms between the two varieties.It lays a foundation for further revealing the material basis underlying the differences in taste,flavor,and the wind-dispersing and heat-clearing efficacy of the two varieties,as well as for clarifying the regulation of key enzyme genes on differential terpenoids.The results provide a reference for the genetic improvement and high-value utilization of Chrysanthemi Flos germplasm resources.关键词
菊花/萜类合成基因/代谢组/转录组Key words
Chrysanthemi Flos/genes involved in terpenoid biosynthesis/metabolome/transcriptome引用本文复制引用
李志洁,杨丹,王星文,陈竹荫,程慧婷,师玉华,陈莎,朱晶晶,王智民..代谢组联合转录组分析探讨不同品种菊花萜类成分差异形成机制[J].中国中药杂志,2026,51(9):2506-2514,9.基金项目
国家自然科学基金项目(82474076) （82474076）
国家重点研发计划项目(2023YFC3504000,2024YFC3506600) （2023YFC3504000,2024YFC3506600）
北京市自然科学基金项目(7242248) （7242248）
