中国免疫学杂志2026，Vol.42Issue(6)：1281-1286,6.DOI:10.3969/j.issn.1000-484X.2026.06.001
铜绿假单胞菌注射液对健康供者免疫功能的影响
Impact of PA-MSHA on immune function of healthy donors
摘要
Abstract
Objective:To investigate impact of Pseudomonas aeruginosa-mannose sensitive hemagglutination(PA-MSHA)on immune status of healthy donors and to provide a theoretical foundation for enhancing immunity in sub-healthy individuals.Methods:A total of 15 healthy donors were recruited from the First Affiliated Hospital of Zhengzhou University and received subcutaneous injec-tions of PA-MSHA.Peripheral blood samples were collected prior to injection and 30 days post-injection,flow cytometric analysis was used to assess proportions of immune cell subsets,expressions of T cell surface activation and inhibition markers,as well as capacity of T cells and NK cells to secrete pro-inflammatory cytokines.High-throughput sequencing was employed to analyze T cell receptor(TCR)diversity.Results:Following PA-MSHA administration,there was a notable increase in CD4/CD8 among participants.Concur-rently,an elevation in activating marker expressions was observed alongside a reduction in inhibitory marker expressions on T cells.Furthermore,both T cells and NK cells exhibited significantly enhanced secretion capabilities for pro-inflammatory cytokines such as IFN-γ,Granzyme B and Perforin;additionally,an increase in peripheral blood TCR diversity was noted.In vitro experiments demon-strated that CD3+T cells treated with PA-MSHA displayed markedly improved functionality compared to control groups.Conclusion:PA-MSHA substantially enhances immune status of healthy donors and offers a viable therapeutic approach for bolstering immunity in sub-healthy populations.关键词
铜绿假单胞菌注射液/CD4/CD8/促炎细胞因子/TCR多样性/免疫功能Key words
PA-MSHA/CD4/CD8/Pro-inflammatory cytokines/TCR diversity/Immune function分类
医药卫生引用本文复制引用
李俊茹,黄建敏,王粉,周凌霄,张震,张毅..铜绿假单胞菌注射液对健康供者免疫功能的影响[J].中国免疫学杂志,2026,42(6):1281-1286,6.基金项目
国家自然科学基金联合基金重点支持项目(U24A20734) （U24A20734）
国家重点研发计划政府间国际科技创新合作重点专项(2022YFE0141000) （2022YFE0141000）
中原学者工作站资助项目(244400510018). （244400510018）
