中国免疫学杂志2026，Vol.42Issue(6)：1403-1408,6.DOI:10.3969/j.issn.1000-484X.2026.06.020
冬凌草甲素调节Ikk/NF-κB信号通路对腰椎间盘退变大鼠髓核细胞凋亡的影响
Impact of oridonin on nucleus pulposus cell apoptosis in rats with lumbar disc degeneration by regulating Ikk/NF-κB signaling pathway
摘要
Abstract
Objective:To investigate the impact of oridonin(ORI)on apoptosis of nucleus pulposus cells in rats with lumbar disc degeneration by regulating the Ikk/NF-κB signaling pathway.Methods:Rat model of lumbar intervertebral disc degeneration was established by subcutaneous injection of D-neneneba galactose.The successfully prepared rats were randomly separated into model group,ORI-L group(5 mg/kg ORI),ORI-M group(10 mg/kg ORI),ORI-H group(20 mg/kg ORI)and ORI-H+Ikkβ activator Pros-tatin group(20 mg/kg ORI+0.5 mg/kg Prostatin)and rats injected with equal volume of physiological saline were as normal group,with 10 rats in each group;after the intervention,ELISA was applied to detect levels of inflammatory factors IL-1β,IL-10 and TNF-α in serum of rats in each group;lumbar intervertebral disc tissue of rats was taken,HE and safranine O staining were applied to detect pathological changes in lumbar intervertebral disc tissue;TUNEL staining was applied to detect changes in apoptosis of nucleus pulposus cells in tissues;qRT-PCR was applied to detect changes in extracellular matrix markers MMP13 and ADAMTS4 mRNA in tissues;Western blot was applied to detect expressions of p-Ikkβ,Ikkβ,NF-κB p65,p-IkB,IkB proteins.Results:Compared with normal group,model group showed shrinkage or disappearance of nucleus pulposus and gaps in fibrous ring,levels of TNF-α and IL-1β in rat serum,pathological score,apoptosis rate of nucleus pulposus cells,MMP13,ADAMTS4 mRNA expressions,p-Ikkβ/Ikkβ,NF-κB p65,p-IkB/IkB expressions in lumbar disc tissue were obviously increased,while level of IL-10 was obviously decreased(P<0.05);compared with model group,pathological damage in ORI-L,ORI-M and ORI-H groups improved,and the quantitative indexes were opposite(P<0.05);Prostratin reversed effect of ORI-H on the above indexes(P<0.05).Conclusion:ORI improves nucleus pulposus cell apoptosis in rats with lumbar disc degeneration by inhibiting Ikk/NF-κB signaling pathway.关键词
冬凌草甲素/Ikk/NF-κB信号通路/腰椎间盘退变/髓核细胞Key words
Oridonin/Ikk/NF-κB signaling pathway/Lumbar disc degeneration/Nucleus pulposus cells分类
医药卫生引用本文复制引用
陈志鹏,段波..冬凌草甲素调节Ikk/NF-κB信号通路对腰椎间盘退变大鼠髓核细胞凋亡的影响[J].中国免疫学杂志,2026,42(6):1403-1408,6.基金项目
武汉市医学科研项目(WZ19C17). （WZ19C17）
