安徽医科大学学报2026，Vol.61Issue(5)：795-802,8.DOI:10.19405/j.cnki.issn1000-1492.2026.05.001
外泌体内miR-320c在姜酚抵御金葡菌感染中的作用
Role of exosomal miR-320c in gingerol-mediated defense against Staphylococcus aureus infection
摘要
Abstract
Objective To investigate the inhibitory effect of gingerol,an active component of ginger,on Staphylo-coccus aureus(S.aureus)infection,and to preliminarily explore its mechanism related to extracellular vesicles(EVs).Methods S.aureus infection models were established in human umbilical vein endothelial cell(HU-VECs),Vero E6 cells,and C57BL/6 mice.Experimental groups included control,infection,and gingerol-treated groups.Bacterial load and VE-cadherin protein expression were detected using immunofluorescence and Western blot.EVs were isolated by size exclusion chromatography and characterized by transmission electron microscopy and nanoparticle tracking analysis.miRNA sequencing was performed on EVs.Results Gingerol treatment signifi-cantly reduced the bacterial load in both in vitro and in vivo infection models and upregulated VE-cadherin expres-sion.miRNA sequencing of EVs revealed that S.aureus infection upregulated the expression of hsa-miR-320c,while gingerol treatment reversed this abnormal expression.Bioinformatic analysis further predicted that the target genes of hsa-miR-320c were significantly enriched in cell junction-related pathways.Conclusion Gingerol exhib-its clear antibacterial and host-protective effects,by regulating hsa-miR-320c in EVs to maintain endothelial bar-rier integrity.关键词
金黄色葡萄球菌/姜酚/外泌体/内皮屏障/VE-cadherinKey words
Staphylococcus aureus/gingerol/extracellular vesicles/endothelial barrier/VE-cadherin分类
医药卫生引用本文复制引用
邢振财,徐萌雪,孔翔,孙婧菡,马震,高宇,杜思远,郑红,刘亚坤..外泌体内miR-320c在姜酚抵御金葡菌感染中的作用[J].安徽医科大学学报,2026,61(5):795-802,8.基金项目
国家自然科学基金项目(编号:82202489) （编号:82202489）
安徽省高校科学研究项目(编号:KJ2021A0215) （编号:KJ2021A0215）
安徽医科大学研究生科研与实践创新项目(编号:YJS20230101) （编号:YJS20230101）
安徽医科大学临床医学("5+3"一体化)专业"早期接触科研"训练计划项目(编号:2021-ZQKY-163、2022-ZQKY-190、2023-ZQKY-131、2024-ZQKY-123) National Natural Science Foundation of China(No.82202489) （"5+3"一体化）
Natural Science Research Project of Anhui Educational Committee(No.KJ2021A0215) （No.KJ2021A0215）
Graduate Student Scientific Research and Practical Innovation Project of Anhui Medical University(No.YJS20230101) （No.YJS20230101）
"Early Exposure to Research"Training Pro-gram for Clinical Medicine("5+3"Integration)at Anhui Medical University(Nos.2021-ZQKY-163,2022-ZQKY-190,2023-ZQKY-131,2024-ZQKY-123) （"5+3"Integration）
