安徽医科大学学报2026，Vol.61Issue(5)：827-835,9.DOI:10.19405/j.cnki.issn1000-1492.2026.05.005
和厚朴酚通过调节ATF4/CHOP/TRIB3通路抑制胃癌细胞恶性进程
Honokiol inhibits the malignant progression of gastric cancer cells by regulating the ATF4/CHOP/TRIB3 pathway
摘要
Abstract
Objective To investigate the effect of honokiol on proliferation,apoptosis,migration,and invasion of gastric cancer cells and its underlying mechanistic.Methods Human gastric cancer cell lines HGC-27 and AGS were treated with Honokiol at concentrations of 0,15,and 25 μmol/L.CCK-8 assays were conducted to determine the half maximal inhibitory concentration(IC50)for both cell lines.Cell viability,proliferation,migration,and in-vasion capabilities were assessed using CCK-8,colony formation,wound healing,Transwell migration and Tran-swell invasion assays.Apoptosis rates were measured via flow cytometry.Western blot analysis examined proteins related to proliferation,apoptosis,migration,invasion,and the endoplasmic reticulum stress pathway ATF4-CHOP-TRIB3.Results Compared with the control group,treatment with 15 and 25 μmol/L Honokiol significantly reduced the proliferation,colony formation,migration,and invasion capabilities of the two gastric cancer cell lines,while significantly increasing the apoptosis rate(P<0.05).Additionally,compared to the control group,the protein expression levels of neural cadherin(N-cadherin),Vimentin,proliferating cell nuclear antigen(PCNA),and B-cell lymphoma/leukemia-2 protein(Bcl-2)decreased in the two gastric cancer cell lines after treat-ment with 15 and 25 μmol/L Honokiol,while the protein expression levels of epithelial cadherin(E-cadherin),Bcl-2-associated X protein(Bax),activating transcription factor 4(ATF4),endoplasmic reticulum stress-related pro-tein(CHOP),and tribbles homolog 3(TRIB3)increased(P<0.05).Conclusion Honokiol promotes apoptosis and inhibits proliferation,migration,and invasion of gastric cancer HGC-27 and AGS cells by regulating the ERS sig-naling pathway ATF4/CHOP/TRIB3.关键词
和厚朴酚/胃癌/内质网应激/ATF4/CHOP/TRIB3信号通路/凋亡/靶向Key words
honokiol/gastric cancer/endoplasmic reticulum stress/ATF4/CHOP/TRIB3 signaling pathway/apoptosis/targeting分类
医药卫生引用本文复制引用
代凯红,文贤慧,黄赟,韦四喜,黄海..和厚朴酚通过调节ATF4/CHOP/TRIB3通路抑制胃癌细胞恶性进程[J].安徽医科大学学报,2026,61(5):827-835,9.基金项目
国家自然科学基金项目(编号:82560798) （编号:82560798）
贵州省卫生健康委科学技术基金项目(编号:gzwjkj2020-1-242) National Natural Science Foundation of China(No.82560798) （编号:gzwjkj2020-1-242）
Scientific and Technological Project of Guizhou Health Commission(No.gzwkj2020-1-242) （No.gzwkj2020-1-242）
