安徽医科大学学报2026，Vol.61Issue(5)：855-860,6.DOI:10.19405/j.cnki.issn1000-1492.2026.05.008
血清中lncRNA H19和miR-22-3p对急性心肌梗死患者的诊断价值
Diagnostic value of serum lncRNA H19 and miR-22-3p in patients with acute myocardial infarction
摘要
Abstract
Objective To explore the expression levels of long non-coding RNA(lncRNA)H19 and microRNA-22-3p(miR-22-3p)in the serum of patients with acute myocardial infarction(AMI)and their diagnostic value for AMI.Methods A total of 176 AMI patients were selected as the experimental group and were divided into ST-segment elevation myocardial infarction(STEMI)group(n=95)and non-ST-segment elevation myocardial infarc-tion(NSTEMI)group(n=81)based on their medical history and electrocardiogram.Meanwhile,156 patients with negative angiography during the same period were selected as the control group(CON group).The relative expres-sion levels of lncRNA H19 and miR-22-3p in the serum of the two groups were detected by real-time fluorescence quantitative polymerase chain reaction.The diagnostic value of miR-22-3p and lncRNA H19 in AMI was evaluated by receiver operating characteristic curve(ROC)analysis.The levels of serum cardiac troponin I(cTnI),creatine kinase(CK),creatine kinase MB isoenzyme(CK-MB),and high-sensitivity C-reactive protein(CRP)were de-tected in all study subjects.Spearman correlation analyses were used to evaluate the correlations between lncRNA H19 and miR-22-3p and cTnI,CK,CK-MB and CRP.Results Compared with the control group,the expression level of lncRNA H19 was upregulated and the expression level of miR-22-3p was downregulated in the AMI group(all P<0.01);compared with the NSTEMI group,the expression level of lncRNA H19 was upregulated and the ex-pression level of miR-22-3p was downregulated in the STEMI group(P<0.01).The areas under the ROC curves(AUC)for the diagnosis of AMI by miR-22-3p,lncRNA H19 and their combination were 0.555,0.977,and 0.983,respectively.lncRNA H19 was positively correlated with cTnI,CK,CK-MB and CRP(P<0.05),and negatively correlated with left ventricular ejection fraction(LVEF)(P<0.001);miR-22-3p was negatively corre-lated with cTnI,CK and CRP,and positively correlated with LVEF(P<0.05).Conclusion The expression level of lncRNA H19 is elevated and the expression level of miR-22-3p decreased in AMI patients,and their levels may have potential value as an auxiliary biomarker for AMI diagnosis and cardiac function assessment.关键词
长链非编码RNA/lncRNA H19/miR-22-3p/急性心肌梗死/心功能Key words
long non-coding RNA/lncRNA H19/miR-22-3p/acute myocardial infarction/cardiac function分类
医药卫生引用本文复制引用
赵胜,刘定红,朱梦宇,戎李,成威,高彦琳..血清中lncRNA H19和miR-22-3p对急性心肌梗死患者的诊断价值[J].安徽医科大学学报,2026,61(5):855-860,6.基金项目
安徽省卫生健康科研项目(编号:AHWJ2024Bai30022) （编号:AHWJ2024Bai30022）
安徽省高校自然科学研究项目(编号:2023AH053377) Health Research Project of Anhui Province(No.AHWJ2024Bai30022) （编号:2023AH053377）
Natural Science Re-search Project of Anhui Educational Committee(No.2023AH053377) （No.2023AH053377）
