安徽医科大学学报2026，Vol.61Issue(5)：872-879,8.DOI:10.19405/j.cnki.issn1000-1492.2026.05.010
糖尿病早期小鼠初级视皮层受损与功能异常情况
Early-stage diabetes induces structural damage and functional deficits in the mouse primary visual cortex
摘要
Abstract
Objective To investigate morphological and functional neural network alterations in the primary visual cortex(V1)during early-stage diabetes.Methods 32 SPF male C57BL/6J mice were randomly divided into con-trol and diabetic groups(n=16).Type 1 diabetes was induced by intraperitoneal injection of streptozotocin(STZ),with model validation via oral glucose tolerance test(OGTT)at week 4.Nissl staining was used to assess morpho-logical changes in brain slices of the two groups.Immunohistochemistry was performed to detect the expression of calcium/calmodulin-dependent protein kinase Ⅱ(CaMKⅡ)and somatostatin(SST)in the V1 area.The co-localization density of SST-positive signals on CaMKⅡ+neurons was then quantified by ImageJ software.In vivo electrophysiology was used for studying neuronal firing and functional connectivity.Results Compared with the control mice,nissl staining showed diabetic mice exhibited significant V1 thinning(P=0.02),reduced neuronal density(P=0.01).Immunohistochemistry found decreased SST integral optical density(P=0.02)and elevated mean fluorescence intensity(P=0.01),and lower SST density on CaMKⅡ+neurons in the diabetic group than in the control group.Compared with the control mice,in vivo electrophysiology studies indicated that diabetic mice showed reduced action potential peak-to-trough ratio and the absolute values of slope(P<0.01),as well as impaired functional connectivity,including conditional firing,mutual information,and Granger causality(P<0.000 1).Con-clusion Early diabetes induces V1 structural atrophy accompanied by SSTergic system disruption and multi-level functional degradation from single neurons to neural networks.关键词
糖尿病/初级视皮层/萎缩/神经元/免疫组化/电生理Key words
diabetes/primary visual cortex/atrophy/neuron/immunohistochemistry/electrophysiology分类
医药卫生引用本文复制引用
陈含露,汤浩琼,封利霞..糖尿病早期小鼠初级视皮层受损与功能异常情况[J].安徽医科大学学报,2026,61(5):872-879,8.基金项目
国家自然科学基金项目(编号:82371094) National Natural Science Foundation of China(No.82371094) （编号:82371094）
