中医药临床杂志2026，Vol.38Issue(6)：1234-1242,9.DOI:10.16448/j.cjtcm.2026.0629
黄芩清热除痹胶囊通过调控LncRNA NONHSAT118801.2抑制NF-κB通路改善强直性脊柱炎湿热痹阻证患者免疫炎症反应
Huangqin Qingre Chubi Capsules Improve Immune Inflammatory Response in Patients with Damp-Heat Bi Syndrome of Ankylosing Spondylitis by Regulating LncRNA NONHSAT118801.2 to Inhibit the NF-κB Pathway
摘要
Abstract
Objective:To investigate the mechanism by which serum containing Scutellaria baicalensis Qingre Chubi capsules improves the immune inflammatory response in patients with ankylosing spondylitis and damp-heat obstruction syndrome.Methods:A macrophage model with knockdown and over-expression of NONHSAT118801.2 was construct-ed;the optimal serum concentration and treatment time of HQC were screened using the CCK-8 assay;macrophage markers were detected by flow cytometry;the expression of TNF-α,IL-1β,IL-6,IL-10,Arg1,CXCR5,and BLC-1/CXCL13 were detected by ELISA;the expression of NONHSAT118801.2,IKBα,and NF-κB p65 mRNA in each group was detected by RT-qPCR;and the expression of NKRF,p-IKBα,and p-NF-κB p65 proteins in macrophages were detected by immunofluorescence and Western blotting.Results:CCK-8 assay showed that serum containing 10%HQC had the strongest inhibitory effect on macrophages after 24 hours of intervention.After HQC intervention,CD86 expression was significantly down-regulated and CD163 expression was significantly up-regulated.Transfection with pc DNA3.1-NONHSAT118801.2 promoted CD86 expression and inhibited CD163 expression.In the functional recovery experiment,HQC-containing serum could reverse the effect of over-expression of NONHSAT118801.2 on the increase in CD86 expression and decrease in CD163 expression.After HQC intervention,the expression of IL-10 and ARG1 was significantly increased,while the expression of BLC-1/CXCL13,CXCR5,IL-6,IL-1β,and TNF-α was signifi-cantly decreased.Transfection with pcDNA3.1-NONHSAT118801.2 significantly promoted the expression of BLC-1/CXCL13,CXCR5,IL-6,IL-1β,and TNF-α,and inhibited the expression of IL-10 and ARG1.In the functional recovery experiment,HQC.The effects of reversible over-expression of NONHSAT118801.2 in HQC-containing serum on increased expression of BLC-1/CXCL13,CXCR5,IL-6,IL-1β,and TNF-α,and decreased expression of IL-10 and ARG1;after HQC intervention,the expression of NONHSAT118801.2,IKBα,NF-κB p65 mRNA,and p-IKBα and p-NF-κB p65 proteins were significantly decreased,while NKRF protein expression was significantly increased.After transfection with pcDNA3.1-NONHSAT118801.2,the expression of NONHSAT118801.2,IKBα,NF-κB p65 mRNA,and p-IKBα and p-NF-κB p65 proteins were significantly increased,while NKRF protein expression was significantly decreased.In the functional recovery experiment,reversible overexpression of NONHSAT118801.2 in HQC-containing serum had a significant effect on...The drug HQC affected the expression of NONHSAT118801.2,IKBα,NF-κB p65 mRNA,and p-IKBα,p-NF-κB p65,and NKRF proteins.Conclusion:HQC-containing serum may inhibit the inflammatory response of AS macrophages by down-regulating NONHSAT118801.2 expression,inhib-iting NF-κB signaling pathway activation,and regulating macrophage polarization and inflammatory factor expression.关键词
强直性脊柱炎/湿热痹阻证/黄芩清热除痹胶囊/炎症反应Key words
Ankylosing spondylitis/Damp-heat obstruction syndrome/Scutellaria baicalensis clearing heat and re-lieving pain capsule/Inflammatory response分类
医药卫生引用本文复制引用
黄旦,刘健,虞佳琪,汪元,万磊,张皖东,方妍妍..黄芩清热除痹胶囊通过调控LncRNA NONHSAT118801.2抑制NF-κB通路改善强直性脊柱炎湿热痹阻证患者免疫炎症反应[J].中医药临床杂志,2026,38(6):1234-1242,9.基金项目
国家自然科学基金青年基金(82205054) （82205054）
安徽省高校自然科学基金重点项目(2024AH0551005) （2024AH0551005）
安徽中医药大学2024年临床研究项目(2024YFYLCZX02) （2024YFYLCZX02）
