北京林业大学学报2026，Vol.48Issue(6)：59-70,12.DOI:10.12171/j.1000-1522.20260162
miR167-XsARF6-IAA模块在文冠果雌蕊败育中的调控机制
Mechanism of the miR167-XsARF6-IAA regulatory module in pistil abortion in Xanthoceras sorbifolium
摘要
Abstract
[Objective]Pistil abortion is a key factor limiting Xanthoceras sorbifolium yield.miR167 may participate in the auxin pathway by regulating ARF genes,but its specific function in the pistil abortion of Xanthoceras sorbifolium remains to be elucidated.This study aimed to investigate the regulatory mechanism of the miR167-XsARF-IAA module during pistil abortion in male flowers of X.sorbifolium Bunge,and to clarify the function of the key IAA pathway gene XsARF6 regulated by miR167,so as to lay a preliminary theoretical foundation for dissecting the regulatory mechanism of pistil abortion in this species.[Method]Pistils of female and male flowers at three key developmental stages were sampled for whole-transcriptome sequencing.The key xso-miR167 was identified by integrated miRNA and mRNA analyses.Biological validation and hormone measurement were performed to analyze the expression patterns of miRNAs and mRNAs were analyzed.IAA content in kernels of X.sorbifolium overexpressing xso-miR167 and phenotypes of transgenic Arabidopsis thaliana were determined to elucidate the dynamic regulation of the miR167-XsARF-IAA module.[Result](1)A total of 481 miRNAs and 2 266 predicted target genes were identified across the three key stages of pistil abortion,with differentially expressed miRNAs showing clear stage-specific patterns,indicating that different miRNAs play critical regulatory roles during the abortion process.(2)The expression of xso-miR167 increased continuously during pistil development in male flowers,significantly repressing its target gene XsARF6.2,and their expression trends were negatively correlated,suggesting that this regulatory pair is a key factor in pistil abortion.(3)xso-miR167 overexpression significantly decreased kernel IAA content compared with wild-type and empty-vector controls.(4)In transgenic A.thaliana,pollen viability decreased from 90.9%in the wild type to 43.68%and 62.25%,stigma diameter and lengths of pistils and stamens were significantly reduced,flowering was delayed,and floral organ development was abnormal.[Conclusion]The miR167-XsARF-IAA regulatory module exists during pistil development in male flowers of X.sorbifolium.miR167 participates in regulating the biosynthesis,metabolism or transport of IAA by inhibiting the expression of XsARF6.2,and plays a key role in pistil abortion of X.sorbifolium.This study provides a new perspective on the molecular mechanism of auxin-regulated pistil development in X.sorbifolium and offers a theoretical basis for fundamental research and breeding applications.关键词
文冠果/雌蕊败育/miR167/生长素响应因子(ARF6)/生长素(IAA)/异源转化/生殖发育/转录后调控Key words
Xanthoceras sorbifolium/pistil abortion/miR167/auxin response factor(ARF6)/indole-3-acetic acid(IAA)/heterologous transformation/reproductive development/post-transcriptional regulation分类
农业科技引用本文复制引用
汪晋竹,敖妍,赵雅欣,陈雨欣,王雪彬,刘阿曼,许叶,郑雅琪,宋文静..miR167-XsARF6-IAA模块在文冠果雌蕊败育中的调控机制[J].北京林业大学学报,2026,48(6):59-70,12.基金项目
国家自然科学基金项目(32572006),国家重点研发计划(2024YFD2201101-6),中央高校基本科研业务费专项(2025XJ04). （32572006）
