北京林业大学学报2026，Vol.48Issue(6)：130-141,12.DOI:10.12171/j.1000-1522.20250447
脱落酸和乙烯利对白桦丛生苗三萜积累及其合成途径基因的调控作用
Effects of abscisic acid and ethephon on triterpenoid accumulation and biosynthetic pathway genes in Betula platyphylla
摘要
Abstract
[Objective]Pentacyclic triterpenoids in birch bark exhibit significant pharmacological activity;however,their natural availability is limited,thereby restricting their further development and utilization.The accumulation of birch triterpenoids is jointly regulated by plant cell engineering technologies and exogenous hormone induction.This study aimed to investigate the regulatory effects of exogenous abscisic acid(ABA)and ethephon on triterpenoid accumulation and the expression of key genes involved in triterpenoid biosynthesis in birch tissue-cultured plantlets.[Method]Clustered birch plantlets were used as experimental materials.Different concentrations of ABA and ethephon were applied on the 7th day of culture.High-performance liquid chromatography(HPLC),colorimetric assays,and quantitative real-time PCR(RT-qPCR)were employed to determine triterpenoid contents,antioxidant enzyme activities,and the expression levels of related genes.[Result](1)Treatment with 10 μmol/L ABA significantly promoted the accumulation of triterpenoids and their components in birch plantlets.The contents of total triterpenoids,betulin,and oleanolic acid reached 82.43,24.61,and 33.13 mg/g,respectively,representing increases of 46.29%,28.73%,and 22.78%compared with the control.Under ethephon treatment at 5 mmol/L,the highest contents of total triterpenoids,betulin,and oleanolic acid were 78.53,26.54,and 35.84 mg/g,respectively,corresponding to increases of 39.07%,54.04%,and 27.02%over the control.(2)Both hormone treatments upregulated the expression of triterpenoid biosynthesis-related genes(HMGR,BPY,and DS)and stress-responsive genes(CYP450 and CP)to varying degrees,with HMGR showing the most pronounced response.In addition,the activities of antioxidant enzymes,including CAT,SOD,APX,and PAL,were significantly enhanced.(3)Key genes in the jasmonic acid(JA)and salicylic acid(SA)signaling pathways were markedly induced by ABA and ethephon treatments.Compared with the control,JAZ1/2 and NPR1 were upregulated,whereas JAZ3/4 and NPR3 were downregulated.Furthermore,important transcription factors in the JA signaling pathway,including MYC2,bHLH44/42,and TGA2/5/7,were significantly upregulated.[Conclusion]Treatment with 10 μmol/L ABA or 5 mmol/L ethephon effectively promoted triterpenoid accumulation in birch plantlets.These treatments also activated the antioxidant defense system and induced the expression of genes involved in JA and SA signaling pathways as well as triterpenoid biosynthesis.This study provides a theoretical basis and technical reference for enhancing triterpenoid production in birch through plant cell engineering approaches.关键词
白桦/脱落酸/乙烯利/三萜类化合物/生物合成基因/JA/SA信号通路Key words
Betula platyphylla/abscisic acid/ethephon/triterpenoids/biosynthetic genes/JA/SA signaling pathway分类
农业科技引用本文复制引用
袁艺格,肖常奕鑫,詹梦燃,陈孟,李尚,詹亚光,尹静..脱落酸和乙烯利对白桦丛生苗三萜积累及其合成途径基因的调控作用[J].北京林业大学学报,2026,48(6):130-141,12.基金项目
国家自然科学基金项目(32371840),黑龙江省自然科学基金重点项目(ZD2023C004),黑龙江春燕创新团队计划(CYCX24023). （32371840）
