草地学报2026，Vol.34Issue(7)：2366-2376,11.DOI:10.11733/j.issn.1007-0435.2026.07.003
星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析
Cloning of PtCASP1 Gene in Puccinellia tenuiflora and Analysis of its Expression Patterns under Saline and Stagnant Stress
摘要
Abstract
Puccinellia tenuiflora is a halophyte with remarkable adaptability to the combined stress of saline and stagnant.The apoplastic barrier formed by endodermal thickening is likely crucial for this adaptation,in which the Casparian strip and suberin lamellae serve as key apoplastic barriers in endodermis for the radial transport of water and solutes.In this study,using Puccinellia tenuiflora as material,we cloned PtCASP1,a key gene involved in Casparian strip formation.Bioinformatic analysis,subcellular localization,and expression pattern analysis under saline,stagnant and combined saline-stagnant stresses were conducted.The results showed that the PtCASP1 gene was 552 bp in length,encoding 184 amino acids.It was a hydrophobic,stable protein and contained cis-acting elements responsive to gibberellin,drought stress,low temperature stress,and light response.Phylogenetic analysis indicated that PtCASP1 was closely related to those in Lolium multiflorum and Alopecurus aequalis.Immunofluorescence analysis revealed that the PtCASP1 protein exhibited dual localiza-tion in the nucleus and cell membrane.The expression level of the PtCASP1 gene in the roots of Puccinellia tenuiflora was significantly higher than that in the leaves,and it responded to saline,stagnant,and combined saline-stagnant stresses,with the highest relative expression levels at 72 hours post-treatment for all stress con-ditions.This study provides a reference for further investigation into the function of the Casparian strip in help-ing Puccinellia tenuiflora adapt to saline-stagnant combined stress.关键词
星星草/PtCASP1/基因克隆/亚细胞定位/表达分析/盐涝复合胁迫Key words
Puccinellia tenuiflora/PtCASP1/Gene cloning/Subcellular localization/Expression analysis/Com-bined saline-stagnant stress分类
农业科技引用本文复制引用
马蓉,杨攀,张蕴文,胡晓炜,王会,王沛..星星草PtCASP1基因克隆及其在盐和涝胁迫下的表达模式分析[J].草地学报,2026,34(7):2366-2376,11.基金项目
四川省科技计划项目(2024NSFSC0310) （2024NSFSC0310）
西南民族大学"双一流"项目(CX2023013) （CX2023013）
西南民族大学青藏高原研究院创新团队项目(2024CXTD11)资助 （2024CXTD11）
