草地学报2026，Vol.34Issue(7)：2450-2460,11.DOI:10.11733/j.issn.1007-0435.2026.07.010
氮磷添加对三江源地区禾豆混播作物根际微生物的影响
Effects of Nitrogen and Phosphorus Addition on Rhizosphere Microorganisms of Leguminous and Gramineae Mixed-Cropped Plants in the Sanjiangyuan Region
摘要
Abstract
To investigate the effect of fertilization on the rhizosphere microorganisms of the barley-pea mixed cropping system in the Sanjiangyuan region,this study took barley(Avena sativa)and forage pea(Pisum sati-vum)as the research objects.A randomized block experiment including nitrogen application(A1),phosphorus application(A2),nitrogen-phosphorus combined application(A3),and no fertilization(CK)was set up.Soil nutrients and root morphology were measured at the milk stage,and microbial communities were analyzed using high-throughput sequencing.The results showed that under the A2 treatment,the ratio of root volume to rhizo-bacteria was 11.15%and 9.49%higher than that under the CK treatment(P<0.05);under the A3 treatment,the root surface area was 63.37%higher than that under the CK treatment(P<0.05).The Venn diagram results indicated that the number of soil fungi under the A1 treatment significantly increased,accounting for 28.89%,compared to 19.12%under the CK treatment;the α diversity index and β diversity index of the A2 treatment indicated that phosphorus fertilizer significantly increased the number of fungi in the soil,and the eco-system tended to be stable;the fungal functional group prediction results showed that after adding nitrogen and phosphorus,the structure and quantity of soil fungi shifted towards saprophytic type.In conclusion,nitrogen and phosphorus fertilization improved the root morphology parameters and soil microbial quantity and structure,and phosphorus fertilizer was more conducive to optimizing the root morphology and microbial community struc-ture of the artificial grassland in the Sanjiangyuan region.关键词
三江源地区/外源肥料添加/人工草地/禾豆混播/根系生态系统Key words
The Sanjiangyuan region/Addition of exogenous fertilizers/Artificial grassland/Mixed sowing of legumes and grasses/Root system ecosystem分类
农业科技引用本文复制引用
冯廷旭,张琳,林伟山,李菲,刘若琪,向雪梅,魏希杰,德科加..氮磷添加对三江源地区禾豆混播作物根际微生物的影响[J].草地学报,2026,34(7):2450-2460,11.基金项目
称多县饲草供给及冷季牲畜利用提质增效技术集成与示范(2024-NK-137) （2024-NK-137）
称多县一年生人工草地养分调控技术研究与示范(2025-NK-P27) （2025-NK-P27）
青海大学三江源生态系统教育部野外科学观测研究站资助 （）
