东北林业大学学报2026，Vol.54Issue(6)：125-133,9.
松材线虫脂肪酸和视黄醇结合蛋白家族的鉴定及表达
Identification and Expression of Fatty Acid and Retinol Binding Protein Families in Bursaphelenchus xylophilus
摘要
Abstract
Eight members of the BxFAR gene family(BxFAR1-BxFAR8)were identified from the genome sequence of B.xylophi-lus.Physicochemical property analysis revealed that the eight BxFAR proteins differed in molecular weight(11.45-21.99 ku),isoelectric point(5.24-10.03),stability,and other characteristics.Among them,BxFAR5 was a hydrophobic pro-tein,while BxFAR8,BxFAR2,and BxFAR3 were unstable proteins.Maximum-likelihood phylogenetic tree of the BxFAR family showed that BxFARs shared high evolutionary similarity in protein motifs with FAR proteins from other nematode species.All eight BxFAR members were dominated by α-helices and random coils,while maintaining high structural con-sistency.Gene expression analysis across different developmental stages suggested that all Bx-far genes,except Bx-far5,were significantly expressed in the fourth-stage juveniles,which may be related to increased energy demand during this stage.Bx-far1,Bx-far2,Bx-far3,Bx-far7,and Bx-far8 were highly expressed in the third-stage juveniles,facilitating nutrient acquisition and suppression of plant immune responses within the host.Bx-far5 was significantly upregulated in dispersal third-stage juveniles(D3),indicating a close association with the diapause behavior of D3 larvae.In summary,the Bx-fars gene family exhibits differential expression profiles across different developmental stages of B.xylophilus dem-onstrated that these genes play important roles in the development and sex differentiation of the pine wood nematode.关键词
松材线虫/脂肪酸和视黄醇结合蛋白/虫龄Key words
Bursaphelenchus xylophilus/Fatty acid and retinol binding protein/Larval age分类
农业科技引用本文复制引用
黄传承,韩东宇,李永红,严涛,唐崟海,马玲,陈洁,郝昕..松材线虫脂肪酸和视黄醇结合蛋白家族的鉴定及表达[J].东北林业大学学报,2026,54(6):125-133,9.基金项目
云南省高等学校大学生创新创业训练计划(S202410677073) （S202410677073）
云南省高原特色农业领域科技计划(202302AE090017) （202302AE090017）
西南林业大学博士科研启动金项目(110223014、110223013) （110223014、110223013）
西南林业大学林学云南省一流建设项目(LXXK-2023M06) （LXXK-2023M06）
黑龙江双一流学科协同创新成果项目(LJGXCG2023-001). （LJGXCG2023-001）
