发光学报2026，Vol.47Issue(6)：1090-1099,10.DOI:10.37188/CJL.20260068
水相中稳定上转换荧光纳米探针的构建及其对硫醇分子的检测
Construction of Stable Upconversion Fluorescent Nanoprobes in Aqueous Phase and Their Detection of Thiol Molecules
摘要
Abstract
Thiol compounds are key molecules for homeostatic regulation and environmental monitoring within bio-logical systems.The development of rapid and highly sensitive thiol detection methods holds significant importance for early disease diagnosis and environmental risk management.Fluorescent probes,owing to their high selectivity,high sensitivity,and non-invasive nature,have emerged as critical tools for detecting bio-small molecules,metal ions,and anions.This study employs IR806-sensitized upconversion nanoparticles(IR806-UCNP)as the core fluo-rescent probe system.By modifying the UCNP surface with polyethyleneimine(PEI),the probe's dispersibility and biocompatibility were effectively enhanced,conferring good hydrophilicity and stability in aqueous environments.Building upon this,the specific nucleophilic substitution reaction between thiol molecules and particular active groups in the IR806 dye was utilized to achieve specific fluorescence signal response of the probe to thiol molecules.This IR806-UCNP upconversion fluorescent probe,excited by near-infrared light,enables rapid and highly sensitive detection of thiol compounds.It exhibits a low detection limit of 0.1 μmol/L for glutathione(GSH),a typical thiol molecule in biological systems,providing a reliable method for subsequent applications in bioanalysis or environmen-tal monitoring.关键词
上转换/荧光探针/近红外/硫醇检测Key words
upconversion/fluorescent probe/near-infrared/thiol detection分类
数理科学引用本文复制引用
邢欢,李新平,张召,鲍国臣,罗玉霞..水相中稳定上转换荧光纳米探针的构建及其对硫醇分子的检测[J].发光学报,2026,47(6):1090-1099,10.基金项目
陕西省自然科学基础研究计划一般项目(2025JC-YBQN-060) （2025JC-YBQN-060）
国家自然科学基金(12004232)Supported by General Project of the Natural Science Basic Research Program of Shaanxi Province(2025JC-YBQN-060) （12004232）
Nation-al Natural Science Foundation of China(12004232) （12004232）
