福建农业学报2026，Vol.41Issue(4)：405-412,8.DOI:10.19303/j.issn.1008-0384.2026.04.001
菌草SRAP-PCR体系优化及引物筛选
SRAP-PCR System Optimization and Primer Selection for Juncao Studies
摘要
Abstract
[Objective]SRAP-PCR system optimization and polymorphic SRAP primers identification for studying Juncao were conducted.[Method]Genomic DNA extracted from Juncao was used as a template in a combined single-factor and orthogonal experimentation to optimize the primer,Mg2+,template DNA,dNTP,and Taq DNA polymerase for SRAP-PCR analysis.A set of polymorphic SRAP primers was selected using 4 varieties of Juncao as targets.[Result]The optimized SRAP-PCR analysis required the concentrations of primer at 1.0 μmol·L-1,Mg2+at 2.50 mmol·L-1,template DNA at 2.5 ng·μL-1,dNTPs at 325 μmol·L-1,and Taq DNA polymerase at 0.35 U.These parameters affected the SRAP amplification efficiency in the order of primer＞template DNA＞Taq DNA polymerase＞dNTPs＞Mg2+.From 35 pairs of SRAP primers,18 with rich polymorphism and clear bands were selected.They had 3 to 10 polymorphic loci with an average polymorphism ratio of 77.36%.[Conclusion]The optimized SRAP-PCR system was highly stable and reliable.It could be applied satisfactorily for analyzing genetic diversity,studying germplasm conservation,and broadening utilization of Juncao.关键词
菌草/SRAP-PCR/体系优化/引物筛选Key words
Juncao/SRAP-PCR/system optimization/primers selection分类
农业科技引用本文复制引用
杨东升,王志鹏,卢倩倩,曹皓然,丁大力,安琪,郝水源..菌草SRAP-PCR体系优化及引物筛选[J].福建农业学报,2026,41(4):405-412,8.基金项目
内蒙古自治区高等学校科学研究重点项目(NJZZ23098) （NJZZ23098）
科技"突围"工程重点项目暨巴彦淖尔国家农高区重大科技项目(2025KJTW04) （2025KJTW04）
巴彦淖尔市科技计划项目(K202108) （K202108）
内蒙古自治区"科技兴蒙"行动重点专项(NMKJXM202201) （NMKJXM202201）
