福建农业学报2026，Vol.41Issue(4)：413-420,8.DOI:10.19303/j.issn.1008-0384.2026.04.002
伪狂犬病病毒感染小鼠脾脏的病理特征及转录组分析
Pathological Observations and Transcriptome Sequencing on Spleens of Pseudorabies Virus-infected Mice
摘要
Abstract
[Objective]Damage on the spleens of mice inoculated with pseudorabies virus(PRV)and pathological regulatory mechanism of the infection were studied,to lay a theoretical foundation for the immune escape mechanism and antiviral targets screening of PRV.[Method]BALB/c mice were inoculated with the PRV variant strain SX-2018,along with healthy ones as control,prior to histopathological observations and transcriptomic sequencing on spleens of the animals.[Result]SX-2018-infected mice showed significant injury on the spleens with the characteristic lymphocyte necrosis in the parenchyma and increased macrophages.The transcriptomic sequencing on the tissue cells identified 299 upregulated and 357 downregulated differentially expressed genes(DEGs).The gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)pathway enrichment analyses indicated that these DEGs were predominantly enriched in the cytokine-cytokine receptor interaction and mitogen-activated protein kinase(MAPK)signaling pathways.Seven representative DEGs were subsequently validated by fluorescence quantitative PCR(qPCR)to yield a highly consistent result with RNA-seq.[Conclusion]The PRV-infected mice exhibited significant structural pathology on spleen with disrupted homeostasis of immune cell subsets.The infection was closely associated with the dysregulated gene expressions in cytokine-cytokine receptor interaction and MAPK signaling pathways of the animal.关键词
猪伪狂犬病病毒/脾脏/病理损伤/转录组测序Key words
pseudorabies virus/mouse spleen/pathological damage/transcriptomic sequencing分类
农业科技引用本文复制引用
赵隆,白瑾琦,魏铭,刘坤,郑小惠,蒙奎洋,辛航阔,朱婷..伪狂犬病病毒感染小鼠脾脏的病理特征及转录组分析[J].福建农业学报,2026,41(4):413-420,8.基金项目
福建省自然科学基金面上项目(2024J01389) （2024J01389）
