福建农业学报2026，Vol.41Issue(4)：461-469,9.DOI:10.19303/j.issn.1008-0384.2026.04.007
不同果肉厚度苦瓜代谢物质鉴定及表达差异分析
Differentially Expressed Metabolites in Momordica charantia of Varied Pulp Thickness
摘要
Abstract
[Objective]Varied pulp thickness and differentially expressed metabolites(DEMs)of Momordica charantia L.germplasms and the associated metabolic pathways and regulatory networks related to the fruit development were analyzed.[Methods]DEMs,KO functional annotation,KEGG pathway enrichment,and expressions of the thick-pulp M.charantia L1 and the thin-pulp L2 at different growth stages were determined to identify the differential metabolites between the two categories of bitter gourds.Comparative and regulatory network analyses based on the enrichment pathways were conducted to further analyze the differentiations.[Results]At all developmental stages,L1 and L2 differed significantly in pulp thickness.For instance,the fruit pulp of L1 was significantly thicker than that of L2 at Stage S3 and S4.There were 511 DEMs found between L1S3(i.e.,L1 at Stage S3,similar for the following)and L1S1,and 545 DEMs between L2S3 and L2S1,with 44%commonly existing in the two comparing groups.The metabolic modules in both groups shown by KO annotation were those most abundantly existed in a category or pathway,while those by KEGG enrichment analysis partially overlapped and in large proportion belonged to"metabolism"-related pathways.The unique DEMs in the groups included dopamine in L1S3 vs.L1S1 and citric acid,D-erythrose-4-phosphate,and L-ornithine in L2S3 vs.L2S1 and participated in 5 or more pathways.Whereas the common DEMs,i.e.,stevioside and pheophorbide A,differed more than 10-fold on FC value.The expressions of those 6 key metabolites were distinctly differentiated,reflecting a significant regulatory variation between L1 and L2.[Conclusion]The thickness of fruit pulp of a M.charantia could serve as an effective visual indicator for quality and market value of the germplasm.The two germplasms of bitter gourds compared in the study showed distinctive DEMs even at different developmental stages,as the plants required specific metabolites to regulate growth,quality,and stress-resistance as they grow.关键词
苦瓜/果肉厚度/代谢物/表达差异Key words
Momordica charantia L./pulp thickness/metabolites/differential expressions分类
农业科技引用本文复制引用
裘波音,温庆放,林珲,张前荣,陈敏氡,郑多乐,李大忠,朱海生..不同果肉厚度苦瓜代谢物质鉴定及表达差异分析[J].福建农业学报,2026,41(4):461-469,9.基金项目
福建省自然科学基金项目(2023J01375) （2023J01375）
福建省瓜果类蔬菜种质资源库(圃)项目(ZYBHDWZX202504) （圃）
国家大宗蔬菜产业技术体系福州综合试验站项目(CARS-23-G51) （CARS-23-G51）
福建省农业科学院优秀科技创新人才专项(YCZX202403) （YCZX202403）
福建省农业高质量发展超越"5511"协同创新工程项目(XTCXGC2021003) （XTCXGC2021003）
