福建农业学报2026，Vol.41Issue(4)：496-506,11.DOI:10.19303/j.issn.1008-0384.2026.04.011
猪粪秸秆翻抛集成堆肥工艺过程中微生物结构和功能演替分析
Microbial Structure and Functional Improvements of Integrated Tossed and Turned Pig Manure/Straw Composting Process
摘要
Abstract
[Objective]By analyzing the succession of microbial community structure and function during the integrated tossing and turning composting process of pig manure and straw,this study aims to elucidate,from a microbiological perspective,the reasons for the advantages of this process in terms of temperature variation and nutrient content.[Method]Two groups were established:Group F,using the integrated tossing and turning composting process of pig manure and straw,and Group G,using natural static composting.Temperature changes and total nutrient content were measured throughout the composting period.The composting progress and product quality of the two groups were compared.Metagenomic analysis was performed to examine differences between Group F and Group G in gene abundance,microbial community structure,metabolic functions,and enzymatic activities,thereby interpreting the observed variations in composting temperature and nutrient content.[Result]Compared with Group G,Group F entered the high-temperature and maturation stages 8 days earlier,had a 6 ℃ higher peak temperature,and showed a 29.53 percentage points higher rise in total nutrient content.Thus,the composting performance and product quality of Group F were markedly better than those of Group G.The numbers of microbial genes found in the F compost at the warming stage(F1),the high-temperature stage(F2),and the cooling stage(F3)were significantly higher than those in the G counterparts(i.e.,G1,G2,and G3,respectively),but lower at the decomposition stage(i.e.,F4 vs.G4).Bacillota,Pseudomonadales,Actinomycetota,Bacteroidete,and Deinococcota were the dominant microbial phylae in the composts.However,they differed on microbiota abundance,as Bacillota existed largely in F1 and F3,while Actinomycetota in G3 and G4;and the enrichment of Actinomycetota occurred gradually in F but only from G2 to G4 in the G compost.The microbial community structure and functions of the composts were highly similar between two adjacent fermentation stages,such as between F2 and G3 and between F3 and G4.[Conclusion]The integrated tossing and turning composting process of pig manure and straw can significantly facilitate the composting process and improve compost quality.The operation enriched microbial population in composts at the first three fermentation stages but reduced that at the 4th stage which encouraged early formation of dominant functional microbial communities of Bacillota and Actinomycetota contributing to the advantages over the conventional practice it generated.关键词
猪粪/秸秆/翻抛集成堆肥/温度/养分含量/微生物群落/功能分析Key words
pig manure/spent straws/tossing and turning over compost pile/temperature/nutrient content/microbial community/functional analysis分类
农业科技引用本文复制引用
成伟伟,赵子惠,周瑶,孙志媛,张鹏岳,李敏,杨小虎,李元新,唐芸娇,杨军祥,杨明,陈伯祥..猪粪秸秆翻抛集成堆肥工艺过程中微生物结构和功能演替分析[J].福建农业学报,2026,41(4):496-506,11.基金项目
甘肃省技术创新引导计划-乡村振兴专项(23CXNL0005) （23CXNL0005）
甘肃省现代丝路寒旱农业科技支撑项目(GSLK-2022-1) （GSLK-2022-1）
