福建畜牧兽医2026，Vol.48Issue(3)：1-4,4.
耐低温降解木质纤维素芽孢杆菌的筛选及性质研究
Screening and characterization of lignocellulose-degrading Bacillus strains with low-temperature tolerance
摘要
Abstract
[Objective]In order to address the issue of low resource utilization efficiency of lignocellulose-like biomass under low-tem-perature conditions,this study was conducted to screen Bacillus strains that can degrade lignocellulose at low temperatures.[Methods]The fresh fecal samples of Protaetia brevitarsis were collected from the breeding base of Protaetia brevitarsis at Xianxian Qiujiang co-operative in Hebei Province.Following the isolation,primary screening,and secondary screening of strains,the obtained isolates were subjected to species identification via morphological analysis,16S rDNA sequence analysis,and physiological and biochemical charac-terization assays.Additionally,their cellulase activities were determined under different temperature and pH conditions.[Results]Five lignocellulose-degrading strains were isolated and screened from the feces of Protaetia brevitarsis,named Bacillus tequilensis N5-2,Bacillus amyloliquefaciens Y5-3,Bacillus subtilis R4-3,Bacillus siamensis N3-5,and Bacillus vallismortis R4-4.The cellulase ac-tivities of R4-3 and R4-4 exhibited a slow decline with decreasing temperature.Specifically,the cellulase activity of R4-3 remained above 80 U/mL even at 20 ℃,and could also reach 80 U/mL at pH 4.0.[Conclusion]Five low-temperature-resistant Bacillus strains that produce lignocellulase were isolated.Among them,Bacillus subtilis R4-3 strain could grow at 10℃and pH 3.5,and grew well at 20℃and pH 4.0,with cellulase activity reaching about 80 U/mL,which provided strain resources for the efficient utilization of agri-cultural and forestry waste in northern winter.关键词
枯草芽孢杆菌/耐低温/木质纤维素酶/白星花金龟Key words
Bacillus subtilis/Low-temperature tolerance/Lignocellulose/Protaetia brevitarsis引用本文复制引用
朱宝成,杨静,余洋,王永攀,郭威,郭晓军..耐低温降解木质纤维素芽孢杆菌的筛选及性质研究[J].福建畜牧兽医,2026,48(3):1-4,4.基金项目
保定市科技计划项目(2494N012)、河北省自然科学基金项目(C2022204227)、河北省中央引导地方科技发展资金项目(226Z2904G)资助. （2494N012）
