分析化学2026，Vol.54Issue(6)：1121-1132,12.DOI:10.19756/j.issn.0253-3820.261028
磁性分子表面印迹聚合物高效富集结合超高效液相色谱-四极杆飞行时间质谱分析复杂基质中胆汁酸类成分
One-pot Synthesis of Magnetic Molecularly Imprinted Polymers Combined with Ultra-high Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry for Efficient Enrichment and Analysis of Bile Acid Components in Complex Systems
摘要
Abstract
A magnetic bile acid surface molecularly imprinted polymer(BA-SMIPs)was synthesized via a one-pot method using deoxycholic acid(DCA)as the template molecule,acrylamide(AM)as the functional monomer,and ethylene glycol dimethacrylate(EGDMA)as the cross-linker,with iron(III)chloride hexahydrate(FeCl3·6H2O)and iron(II)chloride tetrahydrate(FeCl2·4H2O)serving as magnetic sources.The material was structurally characterized by Fourier transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TG),vibrating sample magnetometry(VSM),high-resolution transmission electron microscopy(HRTEM),and X-ray diffraction(XRD).Its adsorption kinetics,isothermal adsorption characteristics,selectivity,and reusability were systematically evaluated.The results demonstrated that under optimized synthesis conditions(Molar ratio:DA∶AM∶EGDMA=1∶4∶30),BA-SMIPs achieved a saturated adsorption capacity of 31.47 mg/g toward the template.The adsorption behavior conformed to the pseudo-second-order kinetic model and the Langmuir isothermal adsorption model,indicating that monolayer chemisorption was the predominant mechanism.Selectivity experiments revealed that the material exhibited significant specific recognition capability for bile acid analogs,with an imprinting factor of 2.64.After eight consecutive adsorption-desorption cycles,the adsorption performance remained 94%of its initial level,showing no significant degradation and demonstrating excellent structural stability and reusability.This method offered advantages including high selectivity,operational simplicity,and reusability,providing a reliable technical reference for the targeted enrichment and analysis of bile acid profiles in complex biological matrices,with promising application potential in biomedical research.关键词
磁性分子表面印迹聚合物/一锅法/超高效液相色谱-四极杆飞行时间质谱/胆汁酸/分析Key words
Magnetic molecularly imprinted polymers/One pot method/Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry/Bile acids/Analysis引用本文复制引用
刘捷,刘帅,何昊奇,于猛,邹忠梅..磁性分子表面印迹聚合物高效富集结合超高效液相色谱-四极杆飞行时间质谱分析复杂基质中胆汁酸类成分[J].分析化学,2026,54(6):1121-1132,12.基金项目
北京市自然科学基金项目(No.7244489)和中国医学科学院医学健康前沿交叉研究项目(No.2023-I2M-QJ-014)资助. Supported by the Beijing Natural Science Foundation(No.7244489)and the CAMS Innovation Fund for Medical Sciences(No.2023-I2M-QJ-014). （No.7244489）
