甘肃医药2026，Vol.45Issue(4)：289-294,305,7.DOI:10.15975/j.cnki.1004-2725.2026.04.001
人参皂苷Rg1对DSS诱导溃疡性结肠炎小鼠肠道屏障及菌群的调控作用
Regulatory effect of ginsenoside Rg1 on intestinal barrier and gut microbiota in mice with DSS-induced ulcerative colitis
摘要
Abstract
Objective:To investigate the regulatory effects and underlying mechanisms of Rg1on intestinal barrier function and gut mi-crobiota homeostasis in mice with DSS-induced UC.Methods:Male SPF-grade C57BL/6 mice were used to establish the DSS-induced UC model,and were randomly divided into the blank control group,model control group,and Rg1 low-dose[50 mg/(kg·d)],medium-dose[100 mg/(kg·d)]and high-dose[200 mg/(kg·d)]groups,with intragastric administration once daily for 14 consecutive days.The body weight,fecal traits and hematochezia of the mice were monitored,and the DAI was calculated.Colon length was measured,colonic pathological damage was evaluated by HE staining,the levels of inflammatory factors in colon tissues were detected by ELISA,and the intestinal microbiota in colonic contents were analyzed via 16S rDNA high-throughput sequencing.Results:Rg1 significantly alleviated DSS-induced body weight loss,re-duced DAI score,and increased colon length in mice(P<0.01).It also markedly down-regulated the levels of IL-1β,IL-6,TNF-α and MPO in colon tissues(P<0.01),attenuated colonic mucosal injury and inflammatory infiltration,increased goblet cell count,elevated intestinal micro-biota α-diversity,restored microbial community structure,corrected abnormal flora abundance at phylum and genus levels,and up-regulated the abundance of beneficial bacteria.Conclusions:Rg1 can effectively alleviate the symptoms and inflammatory response of DSS-induced UC in mice,and its mechanism is closely related to repairing the impaired intestinal barrier and regulating the dysregulated intestinal microbiota.This study provides experimental evidence for the clinical application of Rg1 in UC treatment.关键词
人参皂苷Rg1/结肠炎/肠道菌群/肠道屏障Key words
ginsenoside Rg1/colitis/gut microbiota/intestinal barrier分类
医药卫生引用本文复制引用
王学杰,苏惠,王烁,徐淑娜,王金梦..人参皂苷Rg1对DSS诱导溃疡性结肠炎小鼠肠道屏障及菌群的调控作用[J].甘肃医药,2026,45(4):289-294,305,7.基金项目
济南市卫生健康委员会科技计划项目(编号:2022-2-46) （编号:2022-2-46）
