甘肃中医药大学学报2026，Vol.43Issue(3)：1-8,8.DOI:10.16841/j.issn1003-8450.2026.03.01
基于RhoA/ROCK1信号通路的舒肝和络醒脾方干预肝纤维化模型大鼠作用机制研究
Study on the mechanism of Shugan Heluo Xingpi Fang(舒肝和络醒脾方)intervention in hepatic fibrosis model rats based on the RhoA/ROCK1 signaling pathway
摘要
Abstract
Objective To investigate the mechanism of Shugan Heluo Xingpi Fang(SHXF,舒肝和络醒脾方)against hepatic fibrosis(HF)based on the Ras homolog gene family member A(RhoA)/Rho-associated coiled-coil containing protein kinase 1(ROCK1)signaling pathway.Methods Sixty SPF grade male Wistar rats were random-ly divided into a blank group,a model group,a positive control group,and high,medium,and low dose SHXF groups,with 10 rats in each group.Except for the blank group,the other five groups were intraperitoneally injected with a 40％carbon tetrachloride(CCl4)corn oil solution to establish the HF rat model.During modeling,the blank group received normal saline by gavage,while the other groups received the corresponding drugs by gavage once daily for 8 consecutive weeks.After intervention,serum levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST),albumin(ALB),and globulin(GLB)were measured using an automated biochemical analyzer.Hematoxylineosin(HE)staining and Masson staining were used to observe histopathological changes in liver tissue.Serum levels of hyaluronic acid(HA)and laminin(LN)were detected by enzymelinked immunosorbent assay(ELISA).The relative mRNA and protein expression levels of RhoA and ROCK1 in liver tissue were measured by quantitative reverse transcription polymerase chain reaction(RT-qPCR)and Western blotting,respectively.Results Compared with the blank group,the model group showed significantly increased serum levels of ALT,AST,GLB,HA,and LN,as well as significantly elevated mRNA and protein expression levels of RhoA and ROCK1 in liver tissue,while serum ALB level was significantly decreased(P＜0.05 or P＜0.01).Liver tissue exhibited obvious inflammatory cell infiltration,collagen fiber deposition,and pseudolobule formation,with a significantly in-creased collagen volume fraction(P＜0.01).Compared with the model group,the positive control group and the high and medium dose SHXF groups showed significantly decreased serum levels of ALT,AST,GLB,HA,and LN and significantly increased serum ALB levels(P＜0.05 or P＜0.01).The low dose SHXF group showed significantly re-duced serum levels of ALT,AST,and HA(P＜0.05).Compared with the model group,the positive control group and all SHXF dose groups exhibited alleviated inflammatory cell infiltration and fibrosis in liver tissue,along with signifi-cantly decreased collagen volume fraction and reduced mRNA and protein expression levels of RhoA and ROCK1 in liver tissue(P＜0.05 or P＜0.01).Conclusion Shugan Heluo Xingpi Fang may inhibit hepatic stellate cell activa-tion by regulating the RhoA/ROCK1 signaling pathway,thereby delaying the progression of hepatic fibrosis.关键词
肝纤维化/舒肝和络醒脾方/Ras同源基因家族成员A/Rho相关卷曲螺旋蛋白激酶1信号通路/作用机制/大鼠/实验研究Key words
hepatic fibrosis/Shugan Heluo Xingpi Fang(舒肝和络醒脾方)/Ras homolog gene family member A/Rho-associated coiled-coil containing protein kinase 1 signaling pathway/mechanism of action/rats/experimental study分类
医药卫生引用本文复制引用
陈世玉,赵鲲鹏,张秋菊,王奎淞,李婕妠,郭嘉琪..基于RhoA/ROCK1信号通路的舒肝和络醒脾方干预肝纤维化模型大鼠作用机制研究[J].甘肃中医药大学学报,2026,43(3):1-8,8.基金项目
甘肃省教育科技创新项目(2021CXZX-770). （2021CXZX-770）
