神经损伤与功能重建2026，Vol.21Issue(6)：311-316,6.DOI:10.16780/j.cnki.sjssgncj.20250279
电针"足三里"穴位治疗对重症监护病房获得性衰弱模型大鼠骨骼肌病理变化及炎症因子的影响
Effects of Electroacupuncture at"Zusanli"Acupoint on Skeletal Muscle Pathological Changes and Inflammatory Factors in a Rat Model of Intensive Care Unit-Acquired Weakness
摘要
Abstract
Objective:To observe the effects of electroacupuncture(EA)at the"Zusanli"(ST36)acupoint on the pathology of the gastrocnemius muscle and inflammatory cytokines in a rat model of Intensive Care Unit-acquired weakness(ICU-AW),and to explore its therapeutic effects and potential mechanisms against ICU-AW.Methods:Forty-four male Sprague-Dawley(SD)rats were randomly divided into the control group(n=12),sham operation group(n=12),model group(n=10),and EA group(n=10).The ICU-AW rat model was established in the model and EA groups via cecal ligation and puncture(CLP).The sham operation group underwent laparotomy for exploration followed by closure.After successful modeling,the EA group received daily EA stimulation at bilateral Zusanli acupoints for 30 minutes per session,once a day,for 7 consecutive days.The survival status of rats in each group was observed post-operation.The rats were sacrificed on the 7th postoperative day to collect gastrocnemius muscle and serum samples.Body weight,gastrocnemius wet weight,and the sarcopenia index(SI)were measured.Pathological changes in the gastrocnemius muscle were observed using Hematoxylin and Eosin(HE)staining and Masson staining,while the muscle fiber cross-sectional area(CSA)and collagen volume fraction(CVF)were calculated.The levels of interleukin-1 β(IL-1 β),IL-6,and C-reactive protein(CRP)in the serum and gastrocnemius tissue were detected using enzyme-linked immunosorbent assay(ELISA).Results:(1)Compared with the sham operation group,the model group rats exhibited a poor general condition,weak resistance,and a high mortality rate(P＜0.05).Their body weight,gastrocnemius wet weight,SI,and CSA were significantly decreased(P＜0.05),while the levels of IL-1 β,IL-6,CRP,and CVF in the serum and gastrocnemius tissue were significantly increased(P＜0.05).Pathological examination revealed muscle fiber atrophy and massive accumulation of collagen fibers in the gastrocnemius muscle.(2)Compared with the model group,the EA group showed an increased survival rate(P＜0.05).The body weight,gastrocnemius wet weight,SI,and CSA in the EA group were higher than those in the model group(P＜0.05),while the levels of IL-1 β,IL-6,CRP,and CVF in the serum and gastrocnemius tissue were lower than those in the model group(P＜0.05).The degree of gastrocnemius muscle injury and collagen fiber accumulation in the EA group was lower than that in the model group.Conclusion:Electroacupuncture at the"Zusanli"acupoint may help delay gastrocnemius muscle atrophy in ICU-AW model rats.The mechanism may be related to the inhibition of inflammatory cytokine release and the reduction of collagen fiber formation.关键词
电针/足三里/获得性衰弱/肌肉萎缩/炎性细胞因子Key words
electroacupuncture/Zusanli/ICU-acquired weakness/muscle atrophy/inflammatory cytokines分类
医药卫生引用本文复制引用
胡永胜,刘维琴,高宇,伍柏灵..电针"足三里"穴位治疗对重症监护病房获得性衰弱模型大鼠骨骼肌病理变化及炎症因子的影响[J].神经损伤与功能重建,2026,21(6):311-316,6.基金项目
贵州省科技计划项目[基于"细胞因子风暴"探讨IL-22IL-22R1轴免疫调节脓毒症ICU获得性衰弱的机制及床旁超声评估的研究,No.黔科合支撑(2021)一般 091] （2021）
贵州省中医药、民族医药科学技术研究课题(基于"治痿独取阳明理论"探讨 TGF-βMAPK 轴调控ICU获得性衰弱的机制及补中益气汤的干预研究,No.QZYY-2020-017) （基于"治痿独取阳明理论"探讨 TGF-βMAPK 轴调控ICU获得性衰弱的机制及补中益气汤的干预研究,No.QZYY-2020-017）
贵州中医药大学研究生教育创新计划项目(基于miR-181a/TGF-β/MAPK信号轴探讨ICU获得性衰弱的"脾虚肌萎"病机及电针千预机制,No.YCXKYB2026009) （基于miR-181a/TGF-β/MAPK信号轴探讨ICU获得性衰弱的"脾虚肌萎"病机及电针千预机制,No.YCXKYB2026009）
