广西医科大学学报2026，Vol.43Issue(3)：326-337,12.DOI:10.16190/j.cnki.45-1211/r.2026.03.003
白骨壤叶片细胞外囊泡促进糖尿病创面愈合的作用研究
Effects of Avicennia marina leaf-derived extracellular vesicles on promoting diabetic wound healing
摘要
Abstract
Objective:To investigate the effects of Avicennia marina leaf-derived extracellular vesicles(AmLEVs)on skin wound healing in diabetic rats and explore the underlying mechanisms.Methods:Extracellular vesicles were isolated from Avicennia marina leaves by differential centrifugation combined with ultracentrifugation.The morphology of the extracellular vesicles was observed by transmission electron microscopy(TEM),and the par-ticle size and zeta potential of AmLEVs were measured by dynamic light scattering(DLS)using a Malvern instru-ment.The antioxidant capacity of AmLEVs was evaluated using DPPH,ABTS,and hydroxyl radical assay kits.Human umbilical vein endothelial cells(HUVECs)and human skin fibroblasts(HSFs)were selected as experi-mental cells,and a high-glucose medium containing 25 mmol/L glucose was used to mimic the diabetic microen-vironment.After co-culture with AmLEVs,the levels of intracellular reactive oxygen species(ROS)were detected using the DCFH-DA probe.Cell viability,cell migration,and angiogenic capacity were assessed by cell counting kit-8(CCK-8)assay,scratch wound assay,and tube formation assay,respectively.The expression levels of VEGF mRNA and VEGF protein were determined by real-time quantitative PCR(RT-qPCR)and immunofluo-rescence staining.In addition,a full-thickness skin wound model was established in streptozotocin-induced dia-betic Sprague-Dawley(SD)rats to evaluate the wound-healing effects of AmLEVs.Results:AmLEVs were suc-cessfully isolated,with a typical cup-shaped morphology of the nanovesicles and an average particle size of ap-proximately 55 nm.In vitro experiments showed that AmLEVs exhibited strong scavenging activities against DPPH,ABTS,and hydroxyl radicals.Moreover,100 μg/mL AmLEVs effectively reduced high glucose-induced ROS accumulation,thereby reversing the inhibitory effects of the hyperglycemic environment on the prolifera-tion,migration,and tube formation of HUVECs,thus enhancing the viability of HSFs.Compared with the control group,the mRNA and protein expression levels of VEGF were decreased in the high-glucose injury group.How-ever,these levels were increased when the high-glucose injury group was treated with 100 μg/mL AmLEVs.In vivo experiments demonstrated that the wound healing rate in the rats of diabetic group was significantly decreased compared with that in the rats of the control group.However,the wound healing rate was significantly increased when the diabetic rats were treated with AmLEVs.HE and Masson staining revealed that AmLEV treat-ment enhanced re-epithelialization and increased collagen deposition compared with the diabetic model group.Furthermore,immunofluorescence staining,RT-qPCR,and western blotting confirmed that VEGF mRNA and protein expressions in skin tissues were downregulated in diabetic rats but were significantly upregulated follow-ing AmLEV treatment.Conclusion:AmLEVs effectively promote diabetic wound healing.The underlying mechanisms may involve modulating cellular oxidative stress,enhancing angiogenesis,and improving cell migra-tion,suggesting that AmLEVs can serve as a potential therapeutic agent for diabetic wound treatment.关键词
白骨壤叶片/细胞外囊泡/糖尿病创面/抗氧化/血管生成/血管内皮生长因子/细胞迁移/人脐静脉内皮细胞Key words
Avicennia marina leaves/extracellular vesicles/diabetic wound healing/antioxidation/angiogenesis/vascular endothelial growth factor/cell migration/human umbilical vein endothelial cells分类
医药卫生引用本文复制引用
覃超振,郑伟灏,黄汉记,许胜,陆真慧,郑立..白骨壤叶片细胞外囊泡促进糖尿病创面愈合的作用研究[J].广西医科大学学报,2026,43(3):326-337,12.基金项目
国家自然科学基金资助项目(82360426) （82360426）
广西重点研发计划资助项目(桂科AB23026049) （桂科AB23026049）
