广西植物2026，Vol.46Issue(5)：737-751,15.DOI:10.11931/guihaia.gxzw202503013
水稻OsACS2及同源基因的结构特征和功能分析
Structural characteristics and functional analysis of rice OsACS2 and its homologous genes
摘要
Abstract
To study the structural characteristics and functions of rice OsACS2 gene in rice development,the physicochemical properties,structure,and phylogenetic relationships of OsACS2 and its homologous proteins in rice,Arabidopsis thaliana,maize,wheat,barley,tomato and potato were analyzed using bioinformatics methods.Rice plants were treated with phytohormones abscisic acid(ABA)and methyl jasmonate(MeJA),and the expression levels of OsACS2 gene in rice roots and leaves were analyzed using real-time PCR.The results were as follows:(1)There were a total of 34 OsACS2 homologous genes found in rice,Arabidopsis thaliana,wheat,tomato,potato,maize and barley.(2)OsACS2 showed a closer relationship with its homologs in maize and wheat.(3)OsACS2 protein and its homologs showed similar subcellular localization,contained a conserved AMP(adenosine monophosphate)-binding domain,and had similar secondary and tertiary structures.(4)The promoter of the OsACS2 gene contained ABA and MeJA responsive elements,ABA treatment increased the expression of OsACS2 in roots and decreased its expression level in leaves,MeJA treatment resulted in a decrease in the expression level of OsACS2 in roots,but an increase in leaves.This study establishes a theoretical basis for further understanding the biological functions of OsACS2 and homologous genes.关键词
OsACS2/线粒体/AMP结合结构域/水稻/茉莉酸/脱落酸Key words
OsACS2/mitochondrion/AMP-binding domain/rice/jasmonic acid/abscisic acid分类
农业科技引用本文复制引用
杨欣月,杜云龙,和月英,杨傲,江丽慧,孙晓茜,朱振豪,万媛媛,郭力维,彭晟..水稻OsACS2及同源基因的结构特征和功能分析[J].广西植物,2026,46(5):737-751,15.基金项目
国家自然科学基金(32260085,32460083) （32260085,32460083）
中央引导地方科技发展资金自由探索类基础研究项目(202407AA110006) （202407AA110006）
国家重点研发计划项目(2023YFD1400204) （2023YFD1400204）
云南省应用基础研究计划重点项目(202301AS070082) （202301AS070082）
云南省中青年学术和技术带头人后备人才计划项目(202205AC160076). （202205AC160076）
