广西植物2026，Vol.46Issue(5)：802-813,12.DOI:10.11931/guihaia.gxzw202502013
辣椒黄化环斑病毒主要功能基因N、NSs、NSm的VIGS体系构建及效率验证
Establishment and efficiency validation of VIGS systems for the main functional genes N,NSs and NSm of chilli yellow ringspot virus
摘要
Abstract
Chilli yellow ringspot virus(CYRSV)causes severe diseases in many economically important plants such as cash crops and horticultural flora,its major functional genes(N、NSs、NSm)are closely related to viral infection.However,no report has described the construction of a virus-induced gene silencing(VIGS)system containing N,NSs,NSm genes to study its pathogenic function.To address this,a VIGS system was constructed for the N,NSs and NSm genes of CYRSV,and the roles of these genes during CYRSV infection were analyzed.Approximately 300 bp target fragments of CYRSV N,NSs and NSm genes were respectively inserted into the pTRV2 silencing vector.The silencing efficiency of each gene was detected under different inoculation modes.The optimal inoculation method was selected and further validated in Nicotiana benthamiana and pepper plants.The copy number of N,NSs and NSm genes was quantified using absolute quantitative real-time PCR(qRT-PCR).The results were as follows:(1)The silencing efficiencies for VIGS system of N,NSs,NSm were 82.07％,87.02％,and 94.39％in N.benthamiana,respectively,and 86.63％,89.22％,83.43％in pepper,respectively,compared with the control group.(2)This study successfully established a VIGS system targeting the N,NSs and NSm genes of CYRSV,which effectively inhibited the copy number of N,NSs and NSm genes in N.benthamiana and pepper,with silencing efficiencies ranging from 82％to 95％.The N,NSs and NSm VIGS system developed in this study provides preliminary materials for future investigations into the pathogenesis of CYRSV and offers a theoretical basis for resistance breeding and environmentally friendly control strategies in the field.关键词
辣椒黄化环斑病毒(CYRSV)/病毒诱导的基因沉默(VIGS)/实时荧光定量PCR/拷贝数/本氏烟/辣椒Key words
chilli yellow ringspot virus(CYRSV)/virus-induced gene silencing(VIGS)/qRT-PCR/copy number/Nicotiana benthamiana/pepper分类
农业科技引用本文复制引用
李妤,陈永对,吴阔,马传智,张洁,张仲凯..辣椒黄化环斑病毒主要功能基因N、NSs、NSm的VIGS体系构建及效率验证[J].广西植物,2026,46(5):802-813,12.基金项目
国家自然科学基金(32360638) （32360638）
云南省科技计划项目基础研究专项青年项目(202301AU070121) （202301AU070121）
云南省科技计划项目基础研究专项面上项目(202501AT070087) （202501AT070087）
云南省科技计划项目基础研究专项重大项目(202501BC070017) （202501BC070017）
云南省种子种业联合实验室项目(202205AR070001). （202205AR070001）
