广西植物2026，Vol.46Issue(5)：892-904,13.DOI:10.11931/guihaia.gxzw202511015
红光调控有柄石韦叶片黄酮代谢的多组学解析
Multi-omics analysis of red-light-regulated flavonoid metabolism in Pyrrosia petiolosa leaves
摘要
Abstract
Light quality is a controllable environmental factor in protected cultivation and can reshape secondary metabolism,thereby affecting the accumulation of active constituents and the quality of medicinal plants.In order to investigate the regulatory effects of red light on flavonoid metabolism in Pyrrosia petiolosa leaves and its molecular mechanism,plants were grown under full-spectrum white light(WL)and monochromatic red light(RL),and leaf metabolomic and transcriptomic profiles were generated using liquid chromatography-mass spectrometry(LC-MS)and RNA sequencing(RNA-seq),followed by integrative analysis to associate differentially accumulated metabolites(DAMs)with differentially expressed genes(DEGs)and to identify key pathways and candidate regulatory nodes based on KEGG enrichment.The results were as follows:(1)Compared with WL,RL significantly increased 109 flavonoid-related metabolites,including 51 flavonols and 34 flavones.(2)The contents of kaempferol glycosides and afzelechin were markedly increased,whereas the contents of naringenin chaicone and sakuranetin were decreased,suggesting that red light may redirect pathway flux and promote a shift in flavonoid composition toward the flavonol branch rather than uniformly elevating all flavonoid subclasses.(3)Transcriptome analysis showed significant upregulation of key structural genes in flavonoid biosynthesis,including F3H,FLS,DFR and LAR,and both DEGs and differential accumulated metabolites were significantly enriched in the flavonoid biosynthesis pathway.In conclusion,red light promotes flavonoid accumulation(particularly flavonols)in P.petiolosa leaves by upregulating key biosynthetic genes and modulating pathway flux distribution.These findings provide a theoretical basis for light-quality-based regulation of secondary metabolism to improve the quality consistency of P.petiolosa and to support precision cultivation under controlled environments.关键词
有柄石韦/光质调控/黄酮生物合成/差异积累代谢物/差异表达基因Key words
Pyrrosia petiolosa/light quality-regulated/flavonoid biosynthesis/differentially accumulated metabolites(DAMs)/differentially expressed genes(DEGs)分类
农业科技引用本文复制引用
鲁璇,张艺璇,徐俪华,蓝奇仙,邱思绮,陈建桦,黄荣韶,李良波,曹科鑫..红光调控有柄石韦叶片黄酮代谢的多组学解析[J].广西植物,2026,46(5):892-904,13.基金项目
国家自然科学基金(32160085). （32160085）
