高原农业2026，Vol.10Issue(3)：277-289,13.DOI:10.19707/j.cnki.jpa.2026.03.001
西藏青稞根部内生拮抗芽孢杆菌的筛选、鉴定及防病促生特性
Screening and Identification of Root Endophytic Antagonistic Bacillus from Xizang Highland Barley and Their Biocontrol and Plant Growth-Promoting Traits
摘要
Abstract
To obtain biocontrol microbial resources,endophytic bacteria were isolated and purified from highland barley roots by the dilution plate method in this study.2 highly efficient antagonistic endophytic strains were screened via the plate confrontation assay.Their antifungal activities were further evaluated using cell-free fermentation filtrates and volatile compounds.Strain identification was performed based on colony morphology,physiological and biochemical characteristics,as well as sequence analysis of the 16S rDNA and gyrB genes.The results showed that the 2 strains exhibited plate inhibition rates of more than 75%against the three pathogenic fungi.In the test of cell-free fermentation filtrates,the inhibition rates of strain ZDR20 against the three pathogens ranged from 79.45%to 84.69%,and those of strain ZDT27 ranged from 75.25%to 92.98%.Both strains had the strongest inhibitory effect on Pyrenophora graminea.In the volatile antifungal test,the inhibition rates of ZDR20 and ZDT27 against Pyrenophora graminea and Bipolaris sorokiniana were 60.04%and 34.46%,respectively.Neither strain showed inhibitory activity against Fusarium graminearum.Extracellular enzyme assays indicated that both strains could produce 5 kinds of extracellular enzymes including glucanase and protease.Strains ZDR20 and ZDT27 were finally identified as Bacillus amyloliquefaciens.In summary,2 excellent antagonistic endophytic bacteria were successfully obtained in this study,which can provide valuable strain resources and a theoretical basis for biological control of crop diseases and the development of biocontrol agents.关键词
芽孢杆菌/内生细菌/拮抗活性/促生测定/菌种鉴定Key words
Bacillus/Endophytic bacteria/Antagonistic activity/Growth promotion test/Strain identification分类
农业科技引用本文复制引用
巩文峰,张凤,兰徐颖,金怀,夏洪艳,马晓丹..西藏青稞根部内生拮抗芽孢杆菌的筛选、鉴定及防病促生特性[J].高原农业,2026,10(3):277-289,13.基金项目
国家自然科学基金(31960002) （31960002）
高校发展改革专项资金植物保护学科建设(YJSXK202605). （YJSXK202605）
