海南师范大学学报（自然科学版）2026，Vol.39Issue(2)：169-176,8.DOI:10.12051/j.issn.1674-4942.2026.02.007
白千层黄酮类化学成分及其抗菌活性
Flavonoid Constituents from Melaleuca leucadendron and Their Antibacterial Activities
摘要
Abstract
This study focused on the isolation,purification,identification,and antimicrobial activity evaluation of flavonoids from the tropical medicinal plant Melaleuca leucadendron Linn.The flavonoid constituents in the ethanol extract of this plant were separated and purified using various chromatographic techniques,including normal-phase silica gel column chromatography Sephadex LH-20 gel column chromatography,and semi-preparative HPLC.The structures of the isolated compounds were identified by NMR and MS techniques.A total of fifteen flavonoids were isolated and identified from M.leucadendron:europetin 3-O-α-L-rhamnopyranoside(1),myricitrin(2),quercetin-3-O-rhamnopyranoside(3),myricetin 3-O-(3′′-O-galloyl)-α-rhamnopyranoside 7-methyl ether(4),myricetin 3-O-(2′′-O-galloyl)-α-rhamnopyranoside 7-methyl ether(5),myricetin 3-O-(2′′-O-galloyl)-a-L-rhamnopyranoside(6),quercetin-3-O-β-D-glucoside(7),(-)-epigallocatechin 3-O-gallate(8),epicatechin gallate(9),(-)-gallocatechin 3-O-gallate(10),8-methyl-5,7-dihydroxydihydroflavonoid(11),pinocembrin(12),quercetin(13),(-)-epigallocatechin(14)and gallocatechin(15).Except for compounds 2 and 13,all other compounds were isolated from the genus Melaleuca for the first time.Additionally,the 1H and 13C NMR data of compound 1 are reported here for the first time.Antimicrobial activity tests showed that compounds 8-10 exhibited significant inhibitory activity against four species of pathogenic bacteria.关键词
白千层/化学成分/黄酮/黄酮苷/抗菌活性Key words
Melaleuca leucadendron/chemical constituents/flavonoids/flavonoid glycoside/antibacterial activity分类
医药卫生引用本文复制引用
陈宏洁,吴美珠,林青青,周学明..白千层黄酮类化学成分及其抗菌活性[J].海南师范大学学报（自然科学版）,2026,39(2):169-176,8.基金项目
海南省"南海新星"科技创新人才项目(NHXXRCXM202315) （NHXXRCXM202315）
海南省高层次人才项目(225RC752) （225RC752）
