海南师范大学学报（自然科学版）2026，Vol.39Issue(2)：184-191,8.DOI:10.12051/j.issn.1674-4942.2026.02.009
枳实总黄酮的抗炎和抗氧化活性
Anti-inflammatory and Antioxidant Activity of Total Flavonoids in Aurantii Fructus Immaturus
摘要
Abstract
Aurantii Fructus Immaturus has a long history of pharmacy use in China due to its rich biological activity.Ultrasonic-assisted extraction can destroy its cell wall via mechanical vibration,promote solvent penetration,and enhance bioactive component dissolution.In this study,five solvents of different polarities combined with ultrasonic-assisted technology were used to extract total flavonoids from Aurantii Fructus Immaturus.Antioxidant activity was evaluated by superoxide anion,DPPH and ABTS free radical scavenging assays,and ethanol was identified as the optimal extraction solvent.The anti-inflammatory activity results of ethanol extract on RAW264.7 cells indicated that the concentrations of 25,50,and 100 μg/mL of ethanol extract effectively alleviated the inflammation induced by lipopolysaccharide(LPS)in RAW264.7 cells by inhibiting the production of nitric oxide(NO),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6).This study provides potential technical support for the application of ultrasonic-assisted extraction in the quality control of Aurantii Fructus Immaturus and lays an experimental foundation for its use in functional food and anti-inflammatory drug research and development.关键词
枳实/总黄酮/超声辅助乙醇提取/抗炎活性/抗氧化活性Key words
Aurantii Fructus Immaturus/total flavonoids/ultrasound-assisted ethanol extraction/anti-inflammatory activity/antioxidant activity分类
医药卫生引用本文复制引用
童程杰,刘欣博,谌燕,杨会成..枳实总黄酮的抗炎和抗氧化活性[J].海南师范大学学报（自然科学版）,2026,39(2):184-191,8.基金项目
舟山市科技规划项目(2023C13001、2023C31012) （2023C13001、2023C31012）
浙江省新苗人才计划项目(24127000107) （24127000107）
