海南医科大学学报2026，Vol.32Issue(12)：881-889,9.DOI:10.13210/j.cnki.jhmu.20250314.001
γδT细胞通过IL-17调控TGF-β/Smad信号通路促进CD小鼠肠纤维化
γδT cells promote intestinal fibrosis in CD mice by regulating the TGF-β/Smad signaling pathway through IL-17
摘要
Abstract
Objective:To investigate the role and mechanism of γδT cells regulating the transforming growth factor-β(TGF-β)/Smad signaling pathway through interleukin-17(IL-17)in intestinal fibrosis,based on the Crohn's disease(CD)intesti-nal fibrosis model.Methods:A total of twenty-eight male BALB/c mice were randomly picked out.Thereafter,these mice were classified into four separate groups:the control group,the model group,the anti-IL-17A antibody intervention group,and the anti-TCR γ/δ antibody intervention group.Construct a CD intestinal fibrosis model using TNBS/50％alcohol enema and inject corresponding antibodies.Record the weight and disease activity index(DAI)of mice,and after euthanizing the mice in the 7th week,take colon tissue and measure its length.H&E staining was used to observe changes in tissue morphology and structure;Masson staining was used to observe collagen in colon tissue;Immunohistochemical detection of the expression of α-smooth mus-cle actin(α-SMA);ELISA was used to detect the expression levels of IL-17,TGF-β,interferon-γ(IFN-γ),and IL-10;West-ern blot was used to detect α-SMA,connective tissue growth factor(CTGF),type Ⅰ collagen(Collagen Ⅰ),Collagen Ⅲ,and TGF-β/Smad signaling pathway related proteins.Results:Compared to the control group,the model group showed an increase in DAI score and a reduction in colon length;Pathological examination showed severe damage to the colon tissue,and Masson stain-ing revealed a large number of blue stained collagen fibers;The expression of IFN-γ and IL-10 decreased,while the expression of IL-17,TGF-β,α-SMA,CTGF,Collagen Ⅰ,Collagen Ⅲ,TGF-β1,Smad2/3,and p-Smad2/3 showed a significant increase(P<0.05).However,in comparison to the model group,treatment with anti-IL-17A antibody or anti TCR γ/δ antibody signifi-cantly inhibited the changes in various indicators induced by TNBS in mice(P<0.05)and alleviated intestinal fibrosis.Conclu-sion:Gamma delta T cells can promote intestinal fibrosis,and the mechanism of action may be related to the activation of the IL-17 signaling pathway.关键词
克罗恩病(CD)/肠道/纤维化/γδT细胞/IL-17Key words
Crohn's disease(CD)/Intestine/Fibrosis/γδT T cells/IL-17分类
医药卫生引用本文复制引用
李永荣,陈名利,曾祖妮,陈超超,何周桃,蓝程..γδT细胞通过IL-17调控TGF-β/Smad信号通路促进CD小鼠肠纤维化[J].海南医科大学学报,2026,32(12):881-889,9.基金项目
This study was supported by the National Natural Science Foundation of China(81860102,82060102) （81860102,82060102）
High-Level Talent Program of Natural Science Foundation of Hainan Province(821RC1116) 国家自然科学基金(81860102,82060102) （821RC1116）
海南省自然科学基金高层次人才项目(821RC1116) （821RC1116）
