海南医科大学学报2026，Vol.32Issue(12)：925-935,11.DOI:10.13210/j.cnki.jhmu.20250328.001
当归补血汤调控TNF-α/PI3K-AKT/BMP信号通路改善糖尿病性骨质疏松的机制研究
Mechanism study on the regulation of TNF-α/PI3K-AKT/BMP signalling pathway by Danggui Buxue Decoction to improve diabetic osteoporosis
摘要
Abstract
Objective:To investigate the potential targets and mechanisms of action of Danggui Buxue Decoction(DGBXD)for the treatment of diabetic osteoporosis(DOP)based on network pharmacology,molecular docking,and animal experiments.Methods:The active chemical components of DGBXD were screened by a network pharmacology approach to retrieve the targets of DGBXD related to diabetes osteoporosis.Protein-protein interaction(PPI)networks of drug-disease targets were constructed.Validate the interactions between major targets and active chemical components by molecular docking.Using a db/db diabetic mouse model,the potential targets of DGBXD intervention in DOP were validated and the mechanism of action was explored by ELISA,Western blot and pathology.Results:A total of 413 targets for the action of Astragalus and 65 targets for the action of Angelica sinensis were obtained by network pharmacology,and 15 330 genes were retrieved as DOP-related disease targets.The total number of targets of DGBXD for DOP was 202,including 190 from Astragalus and 11 from Angelica sinensis.Network phar-macological enrichment analysis indicated that DGBXD may improve osteogenic abnormalities in DOP by regulating inflammatory factor expression through modulating TNF-α expression and PI3K-AKT signaling pathway.The results of animal experiments showed that compared to the control group,the serum IL-6,IL-8 and TNF-α levels were significantly increased in the model group(P<0.05);the intervention of DGBXD could inhibit the excessive IL-6(P<0.05),IL-8(P<0.05)and TNF-α(P<0.05)expressions in the model group.Pathological results showed that DGBXD could correct the disorders of bone formation caused by abnormal inflammatory response.Western blot results further indicated that compared to the model group,the interven-tion of DGBXD could significantly reduce the protein expression of TNF-α,AKT,phosphorylated AKT,and correct the abnor-mal protein expression of BMP2(P<0.05).Conclusion:DGBXD may inhibit the inflammatory response and enhance the expres-sion of BMP2 by regulating the TNF-α/PI3K-AKT/BMP signaling pathway,thus promoting the synthesis and mineralization of bone matrix,alleviating the high glucose-induced osteogenic inhibition,improving the balance of bone metabolism,and achieving the therapeutic effect on DOP.关键词
糖尿病骨质疏松/网络药理学/分子对接/当归补血汤Key words
Diabetic osteoporosis/Network pharmacology/Molecular docking/Danggui Buxue Decoction分类
医药卫生引用本文复制引用
张芝桐,卢泽声,陈景昕,任悦怡,董航,姜自伟,黄枫..当归补血汤调控TNF-α/PI3K-AKT/BMP信号通路改善糖尿病性骨质疏松的机制研究[J].海南医科大学学报,2026,32(12):925-935,11.基金项目
This study was supported by the National Natural Science Foundation of China(81974575) （81974575）
the National Famous Traditional Chinese Medicine Expert Inheritance Studio Construction Project[Guozhongyao Renjiaohan(2022)No.75] （2022）
the"Double First Class"and High-Level University Discipline Reserve Talent Cultivation Project of Guangzhou University of Traditional Chinese Medicine （）
Guangzhou Municipal School-Enterprise Joint Funding Project(2025A03J3892) 国家自然科学基金(81974575) （2025A03J3892）
全国名老中医药专家传承工作室建设项目[国中医药人教函(2022)75号] （2022）
广州中医药大学"双一流"与高水平大学学科后备人才培育项目 （）
广州市校(院)企联合资助项目(2025A03J3892) （院）
