基于网络药理学和16S rRNA测序探讨黄连解毒汤治疗2型糖尿病的作用机制
The mechanism of action of Huanglian Jiedu Decoction in the treatment of type 2 diabetes mellitus was studied based on network pharmacology and 16S rRNA sequencing
摘要
Abstract
Objective:To investigate the mechanism of action of Huanglian Jiedu Decoction in the treatment of type 2 diabetes mellitus by network pharmacology and 16S rRNA sequencing methods.Methods:The active ingredients and targets of Huanglian Jiedu Decoction were screened in the TCM Systematic Pharmacology Database and Analysis Platform(TCMSP),and the ob-tained targets were imported into the protein database(UniProt)to find the corresponding gene names.Disease targets were col-lected in the human genome database(GeneCards),the online human Mendelian genetic database(OMIM),and the TTD data-base.The intersection targets of drugs and diseases were obtained through the Venn diagram,and the"drug ingredient-disease tar-get"network was constructed by using Cytoscape 3.7.2 software to determine the key active ingredients.Protein-protein interaction(PPI)analysis was performed through the STRING database,and key targets were screened using the plug-in function in Cyto-scape 3.7.2 software.Then,the KEGG enrichment analysis was carried out through the Metascape database,and the"drug component-disease target-signaling pathway"network was constructed to identify the key signaling pathways.Finally,the network pharmacology results were verified by H&E staining and 16S rRNA sequencing.Results:A total of 85 active ingredients and 236 corresponding targets were found in Huanglian Jiedu Decoction,and 1 549 disease targets were found by network pharmacology analysis.There were 133 intersecting targets and 62 corresponding active ingredients.The key active ingredients in Huanglian Jie-du Decoction are quercetin and wogonin,etc.The core targets of the PPI network analysis were protein kinase 1(AKT1),tumor necrosis factor(TNF),interleukin-6(IL-6),and phosphoinositide 3-kinase/protein kinase B(PI3K/Akt)signaling pathways are closely related.Through animal experiments,it was found that compared to the normal group,the colon tissue structure of the rats in the model group was abnormal,the glands were atrophied and disordered,the spacing between some glands was widened,and the goblet cells were significantly reduced.After the intervention of Huanglian Jiedu Decoction,the tissue structure of the co-lon was significantly improved,the glands were arranged neatly,and a large number of goblet cells could be seen.In addition,Huanglian Jiedu Decoction could affect the richness and diversity of intestinal microbiota,increasing the abundance of Firmicutes at the phylum level,decreasing the abundance of Bacteroides,and increasing the abundance of beneficial bacteria such as Lactoba-cillus,Allobaculum and Blautia at the genus level.LEfSe analysis showed that Allobaculum,Lactococcus and Blautia were dif-ferential markers in the treatment of type 2 diabetes mellitus by Huanglian Jiedu Decoction.Conclusion:Huanglian Jiedu Decoc-tion can promote the proliferation of beneficial bacteria,reduce intestinal inflammation,and repair the intestinal barrier to treat type 2 diabetes.关键词
黄连解毒汤/2型糖尿病/网络药理学/16S rRNA测序Key words
Huanglian Jiedu Decoction/Type 2 diabetes mellitus/Network pharmacology/16S rRNA分类
医药卫生引用本文复制引用
韩雪,唐秋梅,王巍,杨光勇,田维毅,何光志..基于网络药理学和16S rRNA测序探讨黄连解毒汤治疗2型糖尿病的作用机制[J].海南医科大学学报,2026,32(12):936-946,11.基金项目
This study was supported by the National Natural Science Foundation of China(82060796) （82060796）
Guizhou Provincial Science and Technology Foundation Project[Qiankehe Foundation(2020)1Y392] （2020）
Guizhou Provincial Administration of Traditional Chinese Medicine Science and Technology Research Project for Traditional Chinese Medicine and Ethnic Medicine(QZYY-2022-005) （QZYY-2022-005）
Guizhou Provincial Science and Technology Innovation Talent Team Construction Project[Qiankehe Platform Talent(2020)5010] （2020）
Guizhou Provincial Development and Reform Commission Engineering Research Center Construction Project[Qian Development and Reform High-Tech(2020)No.896] （2020）
Guizhou Science and Technology Plan Project[Qiankehe Support(2020)4Y187] 国家自然科学基金(82060796) （2020）
贵州省科技科学技术基金[黔科合基础(2020)1Y392] （2020）
贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY-2022-005) （QZYY-2022-005）
贵州省科技创新人才团队[黔科合平台人才(2020)5010] （2020）
贵州省发改委工程研究中心建设项目[黔发改高技(2020)896号] （2020）
贵州省科技计划项目[黔科合支撑(2020)4Y187号] （2020）
