海南医科大学学报2026，Vol.32Issue(12)：947-960,14.DOI:10.13210/j.cnki.jhmu.20250917.002
液体活检中CTC及其簇的精准检测与分离技术研究进展
Advances in precise detection and isolation techniques for CTC and CTC cluster in liquid biopsy
摘要
Abstract
Circulating tumor cell(CTC)and their clustered structure(CTC cluster)play a pivotal role in the process of dis-tant tumor metastasis.However,they are extremely low abundance in peripheral blood,structural fragility,and phenotypic hetero-geneity pose significant challenges for efficient detection via liquid biopsy.Achieving high-efficiency and structure-preserving en-richment of CTC and CTC cluster has become a critical technical bottleneck in the development of clinically applicable liquid biop-sy systems.In recent years,various separation strategies have been proposed to address the phenotypic diversity and structural in-tegrity of CTC and CTC cluster,including approaches based on physical properties,biomarker recognition,and integrated multi-modal techniques such as immunomagnetic beads,density gradient centrifugation,microfluidic chips,and functionalized nanoplat-forms.This review systematically outlines the developmental trajectory of current mainstream enrichment technologies,with a fo-cus on comparing their performance in terms of capture efficiency,structural preservation,throughput capacity,and clinical appli-cability.Special attention is given to their effectiveness in identifying low-expression heterogeneous subpopulations and preserving the intact architecture of CTC clusters.Furthermore,the review explores recent advancements involving novel materials and intel-ligent release mechanisms,highlighting the challenges and opportunities in functional research and clinical translation.The aim is to provide a comprehensive technical reference and theoretical foundation for building sensitive,specific,and practically applicable platforms for the detection of CTC and CTC cluster.关键词
循环肿瘤细胞(CTC)/CTC簇/分离富集/细胞异质性识别/结构完整性保留Key words
Circulating tumor cell(CTC)/CTC cluster/Separation and enrichment/Cellular heterogeneity recognition/Structural integrity preservation分类
医药卫生引用本文复制引用
蒲佳荣,丁丽华,吴拥军,合思甜..液体活检中CTC及其簇的精准检测与分离技术研究进展[J].海南医科大学学报,2026,32(12):947-960,14.基金项目
This study was supported by the National Natural Science Foundation of China(82273685) （82273685）
Hainan Provincial Key Research and Development Program(Hnky2025ZC-7) （Hnky2025ZC-7）
Hainan Medical University Academic Enhancement Support Program(XSTS2025031) 国家自然科学基金(82273685) （XSTS2025031）
海南省高等学校科学研究项目(Hnky2025ZC-7) （Hnky2025ZC-7）
海南医科大学学术提升支撑计划(XSTS2025031) （XSTS2025031）
