河北医学2026，Vol.32Issue(6)：943-948,6.DOI:10.3969/j.issn.1006-6233.2026.06.010
咪达唑仑调控CX3CL1/CX3CR1通路对无镁诱导的神经元炎性损伤的影响
Effects of Midazolam Regulating the CX3CL1/CX3CR1 Pathway on Magnesium-free Induced Neuronal Inflammatory Injury
摘要
Abstract
Objective:To explore the effects of midazolam(MDZ)regulating the CX3C chemokine lig-and 1(CX3CL1)/CX3C chemokine receptor 1(CX3CR1)pathway on magnesium-free induced neuronal in-flammatory injury.Methods:Primary hippocampal neurons were assigned into the Ctrl group(normal cul-ture),the magnesium-free induction group(neurons were treated with magnesium-free extracellular fluid for 3 h),the L-MDZ group(treated with 0.1μmol/L MDZ for 2h followed by magnesium-free external solution for 3h),the M-MDZ group(treated with 1.0μmol/L MDZ for 2h followed by magnesium-free external solu-tion for 3h),the H-MDZ group(treated with 10μmol/L MDZ for 2 h followed by magnesium-free external solution for 3h),and the AZD8797 group(treated with 10μmol/L MDZ+50nmol/L AZD8797 for 2h followed by magnesium-free external solution for 3h).The LDH concentrations of each group were determined.CCK-8 was used to detect neuronal proliferation.Neuronal apoptosis was detected by flow cytometry.The expres-sions of IL-6,IL-1β and TNF-α in neurons were detected by ELISA.WB was conducted to measure the protein expressions of PCNA,FOXM1,PLK1,CX3CL1,CX3CR1,Bim,Cleaved Caspase-3 and MCL-1 in neurons.Results:The apoptosis rate,Bim,Cleaved Caspase-3,LDH,IL-6,IL-1β,and TNF-α in the magnesium-free induction group were higher than those in the Ctrl group,while the A450 value,PCNA,FOXM1,PLK1,MCL-1,CX3CL1,and CX3CR1 were lower than those in the Ctrl group(P<0.05).The apoptosis rate,Bim,Cleaved Caspase-3,LDH,IL-6,IL-1β and TNF-α in the L-MDZ group,M-MDZ group and H-MDZ group were lower than those in the magnesium-free induction group,while the A450 val-ue,PCNA,FOXM1,PLK1,MCL-1,CX3CL1 and CX3CR1 were higher than those in the magnesium-free induction group(P<0.05).The apoptosis rate,Bim,Cleaved Caspase-3,LDH,IL-6,IL-1β and TNF-α in the AZD8797 group were higher than those in the H-MDZ group,while the A450 value,PCNA,FOXM1,PLK1,MCL-1,CX3CL1 and CX3CR1 were lower than those in the H-MDZ group(P<0.05).Conclusion:MDZ inhibits magnesium-free induced neuronal inflammatory injury by activating the CX3CL1/CX3CR1 path-way.关键词
炎性损伤/咪达唑仑/CX3C趋化因子配体1/CX3C趋化因子受体1/无镁Key words
Inflammatory injury/Midazolam/CX3C chemokine ligand 1/CX3C chemokine re-ceptor 1/Magnesium-free引用本文复制引用
武倩,许海峰,周瑞欣,王振云,刘亚妹..咪达唑仑调控CX3CL1/CX3CR1通路对无镁诱导的神经元炎性损伤的影响[J].河北医学,2026,32(6):943-948,6.基金项目
河北省卫生健康委医学科学研究课题计划项目(20241255) （20241255）
