河北医学2026，Vol.32Issue(6)：949-954,6.DOI:10.3969/j.issn.1006-6233.2026.06.011
基于Nrf2-ARE信号通路探讨维立西呱减轻心肌细胞缺氧复氧损伤作用机制
Mechanism of vericiguat Alleviating Hypoxic Reoxygenation Injury of Cardiomyocytes Based on Nrf2-ARE Signaling Pathway
摘要
Abstract
Objective:To explore the mechanism of vericiguat alleviating hypoxic reoxygenation injury of cardiomyocytes based on Nrf2-ARE signaling pathway.Methods:H9C2 cells were routinely cultured and di-vided into control group(routine culture),hypoxic-reoxygenation group(no serum DMEM culture medium+94%N2+5%CO2+1%O2 and 95%air culture),low dose group,medium dose group and high dose group(adding 1μmol/L,3μmol/L and 10μmol/L vericiguat culture,respectively).SOD and MDA activities in H9C2 cells were determined by TBA method,ROS content was detected by flow cytometry,colony formation assay was used to detect H9C2 cell colony formation,scratch assay was used to detect H9C2 cell migration function,and flow cytometry was used to detect H9C2 cell apoptosis.Nrf2 and ARE proteins in H9C2 cells were detected by Western blot.Results:Compared with control group,MDA,ROS and apoptosis rates were increased in hypoxia-reoxygenation group,while GSH,cell colony formation rate,Nrf2 and ARE were de-creased(P<0.05).Compared with hypoxia-reoxygenation group,MDA,ROS and apoptosis rates were de-creased in low dose vericiguat group,while GSH,cell colony formation rate,Nrf2 and ARE were increased(P<0.05);Compared with low dose vericiguat group,MDA,ROS and apoptosis rate were decreased in me-dium dose vericiguat group,while GSH,cell colony formation rate,Nrf2 and ARE were increased(P<0.05).Compared with medium dose vericiguat group,MDA,ROS and apoptosis rates were decreased in high dose vericiguat group,while GSH,cell colony formation rate,Nrf2 and ARE were increased(P<0.05).The scratch test results showed that there was no significant difference in the blank area among all groups at 0h.After 24h,the blank area increased in the hypoxic-reoxygenation group,and decreased in the low,medium and high dose groups of vericiguat,among which the blank area narrowed significantly in the high dose ver-iciguat group,suggesting that vericiguat significatly alleviated hypoxia/reoxygenation-induced cell migration dysfunction.Conclusion:Vericeiquat has positive effect on alleviating hypoxic-reoxygenation injury in cardio-myocytes,and may exert intervention effect by regulating Nrf2-ARE signaling pathway.This finding can pro-vide new ideas and potential therapeutic targets for clinical treatment of myocardial ischemia-reperfusion inju-ry.关键词
心肌细胞/维立西呱/Nrf2/ARE/损伤Key words
Vericiguat/Cardiomyocytes/vericiguat/Nrf2/ARE/Injury引用本文复制引用
张珍,李翩,刘凯,张欢,方媛..基于Nrf2-ARE信号通路探讨维立西呱减轻心肌细胞缺氧复氧损伤作用机制[J].河北医学,2026,32(6):949-954,6.基金项目
国家自然科学基金项目(82004309) （82004309）
