河北医学2026，Vol.32Issue(6)：1011-1016,6.DOI:10.3969/j.issn.1006-6233.2026.06.021
老年帕金森病患者血清HIF-1α和LncRNA BACE1-AS表达变化与临床分期及认知功能障碍的关系研究
Study on the Relationship between Changes in Serum HIF-1α and LncRNA BACE1-AS Expression with Clinical Staging and Cognitive Impairment in Elderly Parkinson's Disease Patients
摘要
Abstract
Objective:To explore the relationship between changes in serum hypoxia inducible factor-1α(HIF-1α)and long non-coding RNA(LncRNA)β-site amyloid precursor protein cleaving enzyme 1(BACE1-AS)expression with clinical staging and cognitive impairment(CI)in elderly Parkinson's disease(PD)patients.Methods:A total of 90 elderly patients with Parkinson's disease(PD)who were treated in our hospital from January 2020 to December 2023 were selected as the PD group.They were separated into stage Ⅰ(n=25),stage Ⅱ(n=28),stage Ⅲ(n=21),and stage Ⅳ+Ⅴ(n=16)based on clinical stag-ing.According to the patient's cognitive function,they were grouped into a CI group(n=42)and a non CI group(n=48).Another 90 patients who underwent outpatient health examinations were made the control group.Fasting venous blood(3-5 mL)was collected from elderly PD patients on the second day of hospitali-zation and from control subjects during their health check-up.Serum was separated by centrifugation.Serum HIF-1α levels were measured using an ELISA kit.Serum LncRNA BACE1-AS expression was detected by quantitative real-time PCR(qRT-PCR).Clinical baseline data such as age,gender,BMI,smoking history,and alcohol consumption history were collected.Multivariate logistic regression was applied to analyze the in-fluencing factors of CI in elderly PD patients.ROC curve was applied to analyze the predictive value of serum HIF-1α and LncRNA BACE1-AS levels for CI in elderly PD patients.Results:Compared with the control group,the serum levels of HIF-1α and LncRNA BACE1-AS in the PD group were significantly higher(P<0.05).The serum levels of HIF-1α and LncRNA BACE1-AS in stage IV+V were significantly higher than those in stage Ⅰ,Ⅱ,and Ⅲ(P<0.05).The levels in stage Ⅲ were significantly higher than those in stageⅠ and Ⅱ(P<0.05).The levels in stage Ⅱ were significantly higher than those in stage I(P<0.05).Com-pared with the non-CI group,the CI group showed a significant increase in disease duration,HIF-1α,and LncRNA BACE1-AS levels,and a significant decrease in years of education(P<0.05).The course of dis-ease,HIF-1α,and LncRNA BACE1-AS were influencing factors for CI in elderly PD patients(P<0.05).The combined prediction of serum HIF-1α and LncRNA BACE1-AS for the occurrence of CI in elderly PD patients was 0.941,with a sensitivity of 92.86%and a specificity of 87.50%,which was better than the indi-vidual prediction of HIF-1α and LncRNA BACE1-AS(Z combination-HIF-1α=2.434,Z combination-LncRNA BACE1-AS=3.045,P=0.015,0.002).Conclusion:The serum levels of HIF-1α and LncRNA BACE1-AS in elderly PD patients are elevated and correlated with clinical stage and CI.The combination of the two has a high predictive potential for CI in elderly PD patients.关键词
老年帕金森病/低氧诱导因子-1α/长链非编码RNA/β分泌酶反义RNA/临床分期/认知功能障碍Key words
Parkinson's disease/Hypoxia inducible factor-1/Long non-coding RNA/β-site amyloid precursor protein cleaving enzyme 1/Clinical staging/Cognitive impairment引用本文复制引用
沈英生..老年帕金森病患者血清HIF-1α和LncRNA BACE1-AS表达变化与临床分期及认知功能障碍的关系研究[J].河北医学,2026,32(6):1011-1016,6.基金项目
福建省自然科学基金资助项目(2021J01872) （2021J01872）
