河北医学2026，Vol.32Issue(6)：1017-1022,6.DOI:10.3969/j.issn.1006-6233.2026.06.022
急性肺水肿患者外周血单个核细胞铁死亡相关基因表达及其与临床预后的关系分析
Expressions of Ferroptosis-Related Genes in Peripheral Blood Mononuclear Cells and Their Relationship with Clinical Prognosis of Patients with Acute Pulmonary Edema
摘要
Abstract
Objective:To explore the expression characteristics of ferroptosis-related genes in peripheral blood mononuclear cells(PBMC)and analyze their relationship with clinical prognosis of patients with acute pulmonary edema.Methods:A total of 186 patients with acute pulmonary edema and 150 healthy controls in the hospital between March 2022 and March 2025 were enrolled as study group and control group,respective-ly.The mRNA levels of ferroptosis-related genes[glutathione peroxidase 4(GPX4),solute carrier family 7 member 11(SLC7A11),long-chain acyl-CoA synthetase 4(ACSL4)]in PBMC in the two groups were de-tected by real-time fluorescence quantitative polymerase chain reaction(qRT-PCR).According to presence or absence of adverse outcomes(all-cause death,re-admission due to acute pulmonary edema)during 6 months of follow-up,patents in study group were divided into good prognosis group(n=132)and poor prog-nosis group(n=54).The differences in ferroptosis-related genes between study group and control group,as well as among patients with different prognosis were compared.The influencing factors of clinical prognosis in patients with acute pulmonary edema were analyzed by Cox risk regression model,and predictive value of fer-roptosis-related genes in poor prognosis of patients with acute pulmonary edema was analyzed by receiver oper-ating characteristic(ROC)curves.Results:The mRNA levels of GPX4 and SLC7A11 in study group were lower than those in control group,while mRNA level of ACSL4 was higher than that in control group(P<0.05).There were significant differences in APACHE Ⅱ score at admission,mRNA levels of GPX4,SLC7A11 and ACSL4 among patients with different prognosis(P<0.05).APACHE Ⅱ score at admission,mRNA lev-els of GPX4 and SLC7A11 in good prognosis group were higher than those in poor prognosis group,while mR-NA level of ACSL4 was lower than that in poor prognosis group.The results of Cox regression analysis showed that increased APACHE Ⅱ score at admission,decreased GPX4 and SLC7A11 mRNA,and increased ACSL4 mRNA were all independent risk factors of poor prognosis in patients with acute pulmonary edema(P<0.05).The results of ROC curves analysis showed that area under the curve(AUC)values of GPX4,SLC7A11 and ACSL4 mRNA for predicting poor prognosis were 0.760,0.873 and 0.710,all showing good predictive effi-ciency.Conclusion:GPX4,SLC7A11 and ACSL4 are abnormally expressed in patients with acute pulmonary edema,and all the three are closely related to prognosis of patients.关键词
急性肺水肿/铁死亡相关基因/溶质载体家族7成员11/谷胱甘肽过氧化物酶4/长链脂酰辅酶A合成酶4Key words
Acute pulmonary edema/Ferroptosis-related gene/Solute carrier family 7 member 11/Glutathione peroxidase 4/Long-chain acyl-CoA synthetase 4引用本文复制引用
古扎拉依,许姗姗,董辉,热孜万古丽·塔西,郭飞..急性肺水肿患者外周血单个核细胞铁死亡相关基因表达及其与临床预后的关系分析[J].河北医学,2026,32(6):1017-1022,6.基金项目
省部共建中亚高发病成因与防治国家重点实验室高原病专项(SKL-HIDCA-2024-GY5) （SKL-HIDCA-2024-GY5）
