河北中医药学报2026，Vol.41Issue(3)：9-14,6.
基于NLRP3/Caspase-1/GSDMD信号通路探讨姜黄素对过氧化氢诱导的子宫内膜上皮细胞焦亡的影响
Effects of Curcumin on Hydrogen Peroxide-induced Pyroptosis of Endome-trial Epithelial Cells Through NLRP3/Caspase-1/GSDMD Signaling Pathway
摘要
Abstract
Objective:To analyze the effects of curcumin on hydrogen peroxide(H2O2)-induced pyroptosis,pro-inflammatory factor release,and the activity of NLRP3/Caspase-1/GSDMD signaling pathway in endometrial epithelial cells,and to explore the potential mechanism of curcumin in protecting endometrial epithelial cells.Methods:Primary human endometrial epithelial cells were isolated and collected,then randomly divided into four groups:normal group,curcumin group,H2O2 model group,and curcumin treatment group.Hoechst/PI fluorescence staining was performed to observe cellular morphological changes,CCK-8 assay to detect the cell viability,LDH detection kit to measure the LDH release rate,Enzymelinked immunosorbent assay(ELISA)to detect the secretion levels of interleukin1β(IL-1β)and interleukin18(IL-18),and Western Blot to analyze the protein expression of NLRP3,Caspase-1,cleaved Caspase-1,GSDMD,and the Nterminal fragment of GSDMD(GSDMD-N).Results:There were no significant differences in all detected indicators between the normal group and the curcumin group(P>0.05).Compared with the normal group,the H2O2 group exhibited significantly decreased cell viability,increased LDH release rate,and elevated secretion levels of IL-1β and IL-18(all P<0.05).Meanwhile,the protein expression levels of NLRP3 and the ratios of cleaved Caspase-1/Caspase-1 and GSDMD-N/GSDMD were significantly upregulated in the H2O2 group(P<0.05).Compared with the H2O2 group,the curcumin treatment group showed significantly increased cell viability,reduced LDH release rate,and decreased secretion of IL-1β and IL-18(all P<0.05).Moreover,curcumin intervention significantly downregulated the expression of NLRP3 and decreased the ratios of cleaved Caspase-1/Caspase-1 and GSDMD-N/GSDMD(all P<0.05).Conclusion:Curcumin may alleviate H2O2-induced pyroptosis and the release of pro-inflammatory factors in endometrial epithelial cells by regulating the NLRP3/Caspase-1/GSDMD signaling pathway,thereby exerting a protective effect on endometrium.关键词
姜黄素/过氧化氢/子宫内膜上皮细胞/焦亡/促炎因子/NLRP3/Caspase-1/GSDMD通路/子宫内膜异位症Key words
Curcumin/H2O2/endometrial epithelial cells/pyroptosis/pro-inflammatory factors/NLRP3/Caspase-1/GSDMD pathway/endometriosis分类
医药卫生引用本文复制引用
宋利华,栗志英,马景红,贾晓楠,冯文娇,单铁英,李俊娇..基于NLRP3/Caspase-1/GSDMD信号通路探讨姜黄素对过氧化氢诱导的子宫内膜上皮细胞焦亡的影响[J].河北中医药学报,2026,41(3):9-14,6.基金项目
河北省自然科学基金资助项目(H2021402003) （H2021402003）
河北省医学科学研究课题计划资助(20250164) （20250164）
