中医学报2026，Vol.41Issue(7)：1548-1555,8.DOI:10.16368/j.issn.1674-8999.2026.07.228
益气活血化痰方对慢性阻塞性肺疾病大鼠肺功能及炎症因子的影响
Effects of Yiqi Huoxue Huatan Formula on Lung Function and Inflammatory Factors in Rats with Chronic Obstructive Pulmonary Disease
摘要
Abstract
Objective:To investigate the effects of Yiqi Huoxue Huatan Formula(YHHF)on lung function and inflammatory factors in rats with chronic obstructive pulmonary disease(COPD).Methods:Forty-six SD rats were randomly divided into a control group(11 rats)and a modeling group(35 rats),the latter of which was used to establish a COPD model through cigarette smoke exposure com-bined with intratracheal instillation of lipopolysaccharide.After model establishment,the rats were randomly divided into a model group,a low-dose YHHF group(YHHF-L,14.94 g·kg-1),a medium-dose YHHF group(YHHF-M,29.88 g·kg-1),and a high-dose YHHF group(YHHF-H,59.76 g·kg-1),with 8 rats in each group.After 30 days of gavage,lung function indicators of the rats were measured,including respiratory frequency(RF),tidal volume(TV),minute volume(MV),peak inspiratory flow(PIF),peak ex-piratory flow(PEF),and expiratory flow at 50%(EF50).ELISA was used to detect the levels of serum interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α)in rats.Bronchoalveolar lavage fluid(BALF)was collected for cell classification and counting.Hematoxylin and eosin(HE)staining and Masson staining were used to observe the pathological morphology of rat lung tissue.Results:Compared with the control group,the model group rats showed decreased lung function parameters including TV,PIF,PEF,MV,and EF50,and increased RF(P<0.05).Serum IL-1β,IL-6,TNF-α levels,as well as total cell count,eosino-phils,neutrophils,lymphocytes,and macrophage counts in BALF were all elevated(P<0.05),and lung tissue showed obvious inflam-matory pathological changes.Compared with the model group,rats in the YHHF-H group showed increased TV,PIF,PEF,MV,EF50,and decreased RF(P<0.05);serum IL-1β and IL-6 levels,and total cell count,eosinophils,neutrophils,lymphocytes,and macro-phage counts in BALF were decreased(P<0.05);lung tissue pathological damage was alleviated,and fibrosis was improved.Conclu-sion:YHHF can improve lung function in COPD rats,reduce levels of inflammatory factors,and alleviate pathological damage in lung tissue.关键词
慢性阻塞性肺疾病/益气活血化痰方/肺功能/炎症因子/大鼠Key words
chronic obstructive pulmonary disease/Yiqi Huoxue Huatan Formula/lung function/inflammation factor/rat分类
医药卫生引用本文复制引用
李蘩漪,金晶,范彩虹,吴玉,何春辉..益气活血化痰方对慢性阻塞性肺疾病大鼠肺功能及炎症因子的影响[J].中医学报,2026,41(7):1548-1555,8.基金项目
新疆维吾尔自治区自然科学基金青年科学基金项目(2022D01C780) （2022D01C780）
